TAZ 表达的评估及其对人脑胶质瘤侵袭和转移的影响。
Evaluation of TAZ expression and its effect on tumor invasion and metastasis in human glioma.
机构信息
Department of Neurosurgery, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
Department of Neurosurgery, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
出版信息
Asian Pac J Trop Med. 2014 Oct;7(10):757-60. doi: 10.1016/S1995-7645(14)60131-0.
OBJECTIVES
To evaluate the expression of TAZ and its role in tumor invasion and metastasis in human glioma.
METHODS
The expression of TAZ protein was measured in 48 samples of surgically resected human glioma and 13 samples of normal brain tissues using immunohistochemistry. TAZ was knocked down by a retrovirus-mediated TAZ shRNA in a glioma cell line, SNB19. Transwell cell migration and invasion assays were used to determine migration and invasion of SNB19 cells.
RESULTS
The positive expression rate of TAZ protein in glioma tissues was significantly higher than that in normal brain tissues (79.2% vs. 15.4%, P<0.001). Furthermore, clinical analysis suggested that the positive expression rate of TAZ protein in poorly differentiated tumor tissues was significantly higher as compared with that in well differentiated tissues (96.0% vs. 60.9%, P<0.01). TAZ was significantly knocked down by TAZ shRNA (P<0.001), and TAZ knockdown significantly reduced cell migration and invasion (P<0.01, respectively) in SNB19 cells.
CONCLUSIONS
TAZ protein overexpression is observed in human glioma and its elevated expression is significantly correlated with poor differentiation. TAZ knockdown prominently reduces cell migration and invasion in SNB19 cells, suggesting that TAZ may play a key role in the initiation and progression of human glioma.
目的
评估 TAZ 在人类脑胶质瘤侵袭和转移中的表达及其作用。
方法
采用免疫组织化学法检测 48 例手术切除的人脑胶质瘤组织和 13 例正常脑组织中 TAZ 蛋白的表达。利用逆转录病毒介导的 TAZ shRNA 敲低脑胶质瘤细胞系 SNB19 中的 TAZ。采用 Transwell 细胞迁移和侵袭实验检测 SNB19 细胞的迁移和侵袭能力。
结果
脑胶质瘤组织中 TAZ 蛋白的阳性表达率明显高于正常脑组织（79.2%比 15.4%，P<0.001）。此外，临床分析表明，低分化肿瘤组织中 TAZ 蛋白的阳性表达率明显高于高分化组织（96.0%比 60.9%，P<0.01）。TAZ shRNA 明显敲低了 TAZ（P<0.001），且 TAZ 敲低显著降低了 SNB19 细胞的迁移和侵袭（P<0.01，分别）。
结论
TAZ 蛋白在人脑胶质瘤中过度表达，其高表达与低分化显著相关。TAZ 敲低显著降低了 SNB19 细胞的迁移和侵袭，表明 TAZ 可能在人类脑胶质瘤的发生和进展中起关键作用。
Zotero 插件