Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
Department of General Surgery, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, China.
Asian Pac J Trop Med. 2014 Oct;7(10):787-91. doi: 10.1016/S1995-7645(14)60137-1.
To explore the effect of Fibulin-5 expression on cell proliferation and invasion in human gastric cancer patients.
Fibulin-5 expression was detected in 56 samples of surgically resected gastric cancer and paired noncancerous tissues using qRT-PCR and immunoblotting. Fibulin-5 was knocked down by Fibulin-5 shRNA in MGC-803 cells, then BrdU cell proliferation and transwell invasion assays were used to determine cell proliferation and invasion.
The level of Fibulin-5 mRNA in gastric cancer tissues was significantly higher as compared with that in normal tumor-adjacent tissues (P<0.05). Otherwise, the level of Fibulin-5 protein in cancer and noncancerous tissues was consistent with mRNA expression (P<0.05). Fibulin-5 protein expression in tumor tissues with poorly differentiated, lymph node metastasis and advanced TNM tumor stage was significantly higher (P<0.05, respectively). Fibulin-5 was obviously knocked down by Fibulin-5 shRNA (P<0.05), and Fibulin-5 knockdown significantly inhibited cell proliferation and invasion in MGC-803 cells (P<0.05, respectively).
The high-expression of Fibulin-5 is associated with the malignant clinicopathologic parameters in gastric cancer and Fibulin-5 knockdown inhibits cell proliferation and invasion in MGC-803 cells, suggesting Fibulin-5 may act as a key factor in the progression of gastric cancer.
探讨 Fibulin-5 表达对人胃癌患者细胞增殖和侵袭的影响。
使用 qRT-PCR 和免疫印迹法检测 56 例手术切除的胃癌及配对非癌组织中 Fibulin-5 的表达。在 MGC-803 细胞中用 Fibulin-5 shRNA 敲低 Fibulin-5，然后用 BrdU 细胞增殖和 Transwell 侵袭实验测定细胞增殖和侵袭。
胃癌组织中 Fibulin-5 mRNA 的水平明显高于正常肿瘤邻近组织（P<0.05）。另外，癌组织和非癌组织中 Fibulin-5 蛋白的水平与 mRNA 表达一致（P<0.05）。在低分化、淋巴结转移和晚期 TNM 肿瘤分期的肿瘤组织中 Fibulin-5 蛋白表达明显升高（P<0.05，分别）。Fibulin-5 shRNA 明显敲低 Fibulin-5（P<0.05），Fibulin-5 敲低显著抑制 MGC-803 细胞的增殖和侵袭（P<0.05，分别）。
Fibulin-5 的高表达与胃癌的恶性临床病理参数相关，Fibulin-5 敲低抑制 MGC-803 细胞的增殖和侵袭，提示 Fibulin-5 可能是胃癌进展的关键因素。
