Sree ChitraTirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Poojapura, Thiruvananthapuram 695 012, Kerala, India.
Sree ChitraTirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Poojapura, Thiruvananthapuram 695 012, Kerala, India.
Carbohydr Polym. 2014 Nov 4;112:235-44. doi: 10.1016/j.carbpol.2014.05.083. Epub 2014 Jun 6.
Alginate based hybrid copolymer hydrogels with unidirectional pore morphology were prepared to achieve synergistic biological performance for cardiac tissue engineering applications. Alginate based hybrid copolymer (ALGP) were prepared using alginate and poly(propylene fumarate) (HT-PPF) units. Different hybrid bimodal hydrogels were prepared by covalent crosslinking using poly(ethylene glycol diacrylate) and vinyl monomer viz acrylic acid, methyl methacrylate, butyl methacrylate and N-N'-methylene-bis-acrylamide and ionic crosslinking with calcium. The morphologically modified hydrogels (MM-hydrogels) with unidirectional elongated pores and high aspect ratio were prepared. MM-hydrogels favour better mechanical properties; it also enhances cell viability and infiltration due to unidirectional pores. However, the crosslinkers influence the fibroblast infiltration of these hydrogels. Synthesis of collagen and fibroblast infiltration was greater for alginate copolymer crosslinked with poly(ethylene glycol diacrylate-acrylic acid (ALGP-PA) even after one month (288%). This hybrid MM-hydrogel promoted cardiomyoblast growth on to their interstices signifying its potent applications in cardiac tissue engineering.
为了实现协同的生物学性能，用于心脏组织工程应用，制备了具有各向异性孔形态的基于藻酸盐的杂化共聚体水凝胶。基于藻酸盐的杂化共聚体（ALGP）是通过藻酸盐和聚（丙交酯-共-富马酸）（HT-PPF）单元制备的。通过使用聚乙二醇二丙烯酸酯和乙烯基单体如丙烯酸、甲基丙烯酸甲酯、甲基丙烯酸丁酯和 N，N'-亚甲基双丙烯酰胺的共价交联和使用钙离子的离子交联，制备了不同的杂化双模态水凝胶。制备了具有各向异性伸长孔和高纵横比的形态改性水凝胶（MM-水凝胶）。MM-水凝胶具有更好的机械性能，由于各向异性孔，还提高了细胞活力和浸润性。然而，交联剂影响这些水凝胶中成纤维细胞的浸润。对于用聚乙二醇二丙烯酸酯-丙烯酸（ALGP-PA）交联的藻酸盐共聚物，即使在一个月后（288%），胶原的合成和成纤维细胞的浸润也更大。这种杂化 MM-水凝胶促进了心肌细胞在其间隙中的生长，表明其在心脏组织工程中有潜在的应用。
