Thankam Finosh Gnanaprakasam, Muthu Jayabalan
Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Thiruvananthapuram 695012, Kerala State, India.
Sree Chitra Tirunal Institute for Medical Sciences and Technology, Polymer Science Division, BMT Wing, Thiruvananthapuram 695012, Kerala State, India.
J Mech Behav Biomed Mater. 2014 Jul;35:111-22. doi: 10.1016/j.jmbbm.2014.03.010. Epub 2014 Apr 12.
Maintaining the mechanical properties of biofunctional hydrogels of natural resources for tissue engineering and biomedical applications for an intended period of duration is a challenge. Though anionic polysaccharide alginate has been hailed for its excellent biomimetic characters for tissue engineering, it usually fails in load bearing and other dynamic mechanical environment. In this paper this issue was addressed by copolymerizing alginate with the biocompatible and mechanically robust synthetic biodegradable polyester and crosslinking with polyethylene glycol diacrylate (PEGDA) and vinyl co-monomers, 2-hydroxy ethyl methacrylate (HEMA), methyl methacrylate (MMA) and N N׳ methylene bis acrylamide (NMBA) to form three hydrogels. All three hydrogels were amphiphilic, hemocompatible and non-cytotoxic. These hydrogels exhibited appreciable water holding capacity. Comparatively, hydrogel prepared with PEGDA-NMBA crosslinkers displayed larger pore size, increased crosslinking, higher tensile strength and controlled degradation. With appreciable swelling and EWC, this hydrogel elicited better biological responses with long-term cell viability for cardiac tissue engineering.
在一段预期的持续时间内，维持用于组织工程和生物医学应用的天然资源生物功能水凝胶的机械性能是一项挑战。尽管阴离子多糖藻酸盐因其在组织工程中出色的仿生特性而备受赞誉，但它通常在承载和其他动态机械环境中表现不佳。在本文中，通过将藻酸盐与生物相容性好且机械性能强大的合成可生物降解聚酯共聚，并与聚乙二醇二丙烯酸酯（PEGDA）和乙烯基共聚单体甲基丙烯酸2-羟乙酯（HEMA）、甲基丙烯酸甲酯（MMA）以及N,N'-亚甲基双丙烯酰胺（NMBA）交联，以形成三种水凝胶，解决了这一问题。所有这三种水凝胶均具有两亲性、血液相容性且无细胞毒性。这些水凝胶表现出可观的保水能力。相比之下，用PEGDA-NMBA交联剂制备的水凝胶显示出更大的孔径、更高的交联度、更高的拉伸强度以及可控的降解性。凭借可观的溶胀和平衡含水率，这种水凝胶在心脏组织工程中引发了更好的生物学反应以及长期的细胞活力。
