利用葡聚糖蔗糖酶和葡聚糖酶的协同催化作用合成不同Mw 的寡葡聚糖。
Biosynthesis of oligodextrans with different Mw by synergistic catalysis of dextransucrase and dextranase.
机构信息
Department of Pharmaceutical Engineering, School of Medical Engineering, HeFei University of Technology, 193 Tunxi Road, Hefei 230009, PR China.
Department of Pharmaceutical Engineering, School of Medical Engineering, HeFei University of Technology, 193 Tunxi Road, Hefei 230009, PR China.
出版信息
Carbohydr Polym. 2014 Nov 4;112:387-95. doi: 10.1016/j.carbpol.2014.06.018. Epub 2014 Jun 17.
In this study, we have investigated various adding times of dextranase to the dextransucrase system to reveal the synergistic process of dextransucrase and dextranase. Dextranase added into the dextransucrase-sucrose system at different times gave rise to different main dextran products. The results showed that dextranase added into sucrose system at the same time with dextransucrase synthesized low molecular weight (Mw) dextran targeted to 5 kDa, while dextranase added during the reaction process of dextransucrase directionally prepared dextran with medium Mw of 10 kDa and 20 kDa. Fourier-transform infrared (FTIR) spectroscopy and (1)H Nuclear magnetic resonance (NMR) exhibited that the synthesized oligodextrans were mainly composed of α-1,6-glycosidic linkages (dextran 6587 Da, 96.82%; dextran 22,521 Da, 96.03%) and low α-1,3-glycosidic branch. The research established the relationship between the synergistic catalysis of the double enzymatic system and the synthesis of oligodextrans with different Mw.
在这项研究中，我们研究了不同添加时间的葡聚糖酶到葡聚糖蔗糖酶体系中，以揭示葡聚糖蔗糖酶和葡聚糖酶的协同作用过程。葡聚糖酶在不同时间添加到葡聚糖蔗糖酶-蔗糖体系中，产生了不同的主要葡聚糖产物。结果表明，葡聚糖酶与葡聚糖蔗糖酶同时添加到蔗糖体系中，合成了分子量为 5 kDa 的低分子量（Mw）葡聚糖，而葡聚糖酶在葡聚糖蔗糖酶定向合成中分子量为 10 kDa 和 20 kDa 的葡聚糖的反应过程中添加。傅里叶变换红外（FTIR）光谱和（1）H 核磁共振（NMR）表明，合成的寡糖主要由α-1,6-糖苷键（葡聚糖 6587 Da，96.82%；葡聚糖 22,521 Da，96.03%）和低α-1,3-糖苷支化组成。该研究建立了双酶体系协同催化与不同 Mw 寡糖合成之间的关系。
Zotero 插件