使用振荡管密度计作为酶动力学研究工具。测定抗坏血酸钠对转化酶、葡聚糖蔗糖酶和葡聚糖酶的影响。
The use of an oscillating-tube densitometer as a tool in enzyme kinetics. Determination of the influence of sodium ascorbate on invertase, dextransucrase and dextranase.
作者信息
Greulich K O, Ludwig H
出版信息
J Biochem Biophys Methods. 1979 Dec;1(6):327-34. doi: 10.1016/0165-022x(79)90023-x.
The use of a commercial oscillating-tube densitometer with an accuracy of 4 . 10(-7) g/cm3 for the determination of enzyme-kinetics constants is tested. This method is applied to the investigation of the influence of vitamin C (sodium ascorbate) on the glycolytic enzymes invertase, dextransucrase and dextranase. Invertase is inhibited uncompetitively, dextransucrase non-competitively. There is no significant effect of the vitamin on dextranase. The comparison of the mechanisms of the three enzymes suggests that only those reaction steps are inhibited by vitamin C in which fructose is released from the enzyme.
测试了使用精度为4×10⁻⁷ g/cm³ 的商用振荡管密度计来测定酶动力学常数。该方法用于研究维生素C（抗坏血酸钠）对糖酵解酶转化酶、葡聚糖蔗糖酶和葡聚糖酶的影响。转化酶受到非竞争性抑制，葡聚糖蔗糖酶受到非竞争性抑制。维生素对葡聚糖酶没有显著影响。对这三种酶作用机制的比较表明，维生素C仅抑制那些从酶中释放果糖的反应步骤。
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