Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, C.P.19046, Curitiba 81531-980, Brazil.
Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná, Londrina 86001-970, Brazil.
Carbohydr Polym. 2014 Nov 4;112:686-94. doi: 10.1016/j.carbpol.2014.06.042. Epub 2014 Jun 26.
Coffea arabica is the most important agricultural commodity in the world, and salinity is a major threat to its sustainable irrigation. Coffee leaf polysaccharides from plants subjected to salt stress were extracted and the leaves visualized through optical and electron microscopy. Alterations were detected in the monosaccharide composition of the pectin and hemicelluloses, with increases in uronic acid in all fractions. Changes in the polysaccharides were confirmed by HPSEC and FTIR. Moreover, the monolignol content was increased in the final residue, which suggests increased lignin content. The cytoplasm was altered, and the chloroplasts appeared irregular in shape. The arrangement of the stroma lamellae was disordered, and no starch granules were present. It was concluded that leaves of C. arabica under salt stress showed alterations in cell wall polysaccharides, increased monolignol content and structural damage to the cells of the mesophyll.
阿拉比卡咖啡是世界上最重要的农业商品，而盐度是其可持续灌溉的主要威胁。从受到盐胁迫的植物中提取咖啡叶多糖，并通过光学和电子显微镜观察叶片。果胶和半纤维素的单糖组成发生变化，所有级分中的酸性多糖增加。多糖的变化通过 HPSEC 和 FTIR 得到证实。此外，最终残渣中的愈创木酚含量增加，表明木质素含量增加。细胞质发生改变，叶绿体形状不规则。基质片层的排列紊乱，没有淀粉颗粒。综上所述，盐胁迫下的阿拉比卡咖啡叶片表现出细胞壁多糖的变化、愈创木酚含量的增加以及叶肉细胞的结构损伤。
