绿色木霉与齐整小核菌在土壤中混合培养时的蛋白水解活性

Proteolytic activity of Trichoderma viride in mixed culture with Sclerotium rolfsii in soil.

作者信息

Rodriguez-Kabana R, Kelley W D, Curl E A

出版信息

Can J Microbiol. 1978 Apr;24(4):487-90. doi: 10.1139/m78-079.

Abstract

Cultures of Sclerotium rolfsii and Trichoderma viride together in autoclaved soil were assayed at intervals during 8 days of incubation for proteolytic activity (PA) of T. viride. Significant proteolytic activity was detected only in soil containing T. viride (i.e., T. viride alone or S. rolfsii + T. viride); greatest activity occurred between 3 and 4 days after infestation and declined rapidly thereafter. Maximal PA in the mixed-culture soil was accompanied by an increase in soil pH. optimal pH values for PA was 5.5-6.5 with a maximum at 6.0.

摘要

在高压灭菌的土壤中，将齐整小核菌和绿色木霉共同培养，并在8天的培养期间定期测定绿色木霉的蛋白水解活性（PA）。仅在含有绿色木霉的土壤中（即单独的绿色木霉或齐整小核菌+绿色木霉）检测到显著的蛋白水解活性；侵染后3至4天活性最高，此后迅速下降。混合培养土壤中的最大PA伴随着土壤pH值的升高。PA的最佳pH值为5.5 - 6.5，在6.0时达到最大值。