Saito Katsuichi, Nakamura Toshihide, Kobayashi Isao, Ohnishi-Kameyama Mayumi, Ichinose Hitomi, Kimura Keitarou, Funane Kazumi
a National Food Research Institute, National Agriculture and Food Research Organization (NARO) , Tsukuba , Japan.
Biosci Biotechnol Biochem. 2014;78(12):2120-7. doi: 10.1080/09168451.2014.948375. Epub 2014 Aug 18.
Some Lactobacillus brevis strains were found to aggregate upon the addition of xylan after screening for lactic acid bacteria that interact with plant materials. The S-layer proteins of cell surface varied among the strains. The strains that displayed xylan-mediated aggregation retained its ability even after the removal of S-layer proteins. L. brevis had negative zeta potentials. A correlation between the strength of aggregation and zeta potential was not observed. However, partial removal of S-layer proteins resulted in decreases in the electric potential and aggregation ability of some strains. Therefore, xylan-mediated aggregation of L. brevis was considered to be caused by an electrostatic effect between the cells and xylan. L. brevis also aggregated in the presence of mucin, and the strengths of aggregation among the strains were similar to that induced by xylan. Thus, xylan- and mucin-mediated L. brevis aggregation was supposed to be caused by a similar mechanism.
在筛选与植物材料相互作用的乳酸菌后，发现一些短乳杆菌菌株在添加木聚糖后会聚集。细胞表面的S层蛋白在不同菌株间存在差异。表现出木聚糖介导聚集的菌株即使在去除S层蛋白后仍保留其聚集能力。短乳杆菌的ζ电位为负。未观察到聚集强度与ζ电位之间的相关性。然而，部分去除S层蛋白会导致一些菌株的电位和聚集能力下降。因此，短乳杆菌的木聚糖介导聚集被认为是由细胞与木聚糖之间的静电作用引起的。短乳杆菌在黏蛋白存在的情况下也会聚集，并且各菌株间的聚集强度与木聚糖诱导的相似。因此，木聚糖和黏蛋白介导的短乳杆菌聚集被认为是由相似的机制引起的。
