Vila Marta M D C, Coelho Sebastiao L, Chaud Marco V, Tubino Matthieu, Oliveira Jose M, Balcao Victor M
LaBNUS - Laboratory of Biomaterials and Nanotechnology of the University of Sorocaba at Sorocaba's Technological Park, University of Sorocaba, Cidade Universitária Prof. Aldo Vannucchi, Rod. Raposo Tavares km 92.5, CEP 18023-000 Sorocaba, São Paulo, Brazil.
Curr Pharm Biotechnol. 2014;15(2):182-90. doi: 10.2174/138920101502140807125254.
The goal of the research work entertained herein was the development and characterization of a poly-(vinyl alcohol) (PVA) hydrogel cross-linked with glutaraldehyde and impregnated with 0.2% (w/w) nitrofurazone (NTZ), for topical applications. To verify the active principle release capability, one has determined (i) swelling profile, (ii) in vitro release of NTZ via UV-VIS spectrophotometry, and (iii) antimicrobial activity via exposure to the hydrogel of ATCC strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The optimized hydrogel was further characterized via scanning electron microscopy (SEM), infrared spectroscopy with Fourier transform, moisture content determinations and thermal analyses via thermal gravimetry (TGA). Swelling tests revealed a mass increase from 100±5% up to 350±11%. Incorporated NTZ displayed bactericidal activity, as expected, being released in a linearly controlled fashion above 6 µg/mL during experiment timeframes of 14 h. SEM analyses allowed verification of a homogeneous surface morphology, while infrared spectra showed that NTZ did not bind strongly to the cross-linked polymer. Furthermore, results from thermal analyses suggested a loss of thermal stability arising from incorporation of NTZ in the hydrogel. The optimized hydrogel exhibited characteristics with high potential for (antimicrobial) treatment of skin lesions.
本文所开展的研究工作目标是开发一种用戊二醛交联并浸渍0.2%（w/w）呋喃西林（NTZ）的聚（乙烯醇）（PVA）水凝胶，用于局部应用。为了验证活性成分释放能力，测定了：（i）溶胀曲线；（ii）通过紫外-可见分光光度法测定NTZ的体外释放；以及（iii）通过将金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的ATCC菌株暴露于水凝胶来测定抗菌活性。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱、水分含量测定以及热重分析（TGA）进行热分析，对优化后的水凝胶进行了进一步表征。溶胀试验显示质量增加从100±5%至350±11%。如预期的那样，掺入的NTZ表现出杀菌活性，在14小时的实验时间内，以线性可控方式在6 µg/mL以上释放。SEM分析证实了表面形态均匀，而红外光谱表明NTZ与交联聚合物的结合不强。此外，热分析结果表明，由于NTZ掺入水凝胶中导致热稳定性丧失。优化后的水凝胶表现出对皮肤损伤（抗菌）治疗具有高度潜力的特性。
