Biological Science Research Center, State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China.
College of Biotechnology, Southwest University, Beibei, Chongqing 400715, China.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:341-351. doi: 10.1016/j.msec.2019.03.111. Epub 2019 Mar 29.
Developing a non-toxic, super-absorbent and antibacterial hydrogel as a skin wound dressing is of significant importance. Sericin hydrogel is an ideal dressing material as it has excellent biocompatibility, biodegradability, moisture retention, high affinity to biomolecules, self-healing and promoting cell proliferation activity. Here, we blended silk sericin (SS) with poly(vinyl alcohol) (PVA) to prepare a SS/PVA hydrogel through repetitive freeze-thawing. The photoluminescence of SS/PVA hydrogel indicated PVA was well blended with sericin. SS/PVA hydrogel showed excellent hydrophilicity and swelling behavior for its porous structure. PVA blending effectively improved the thermostability of sericin and enhanced the mechanical property of sericin, but did not affect the crystallinity of sericin and PVA. SS/PVA hydrogel exhibited the ability to load and release small molecule drugs and silver nanoparticles. Cytotoxicity and immuno-toxicity assays suggested the gentamicin loaded SS/PVA hydrogel had excellent cytocompatibility on mammalian cells. Bacterial inhibition assay and wound infection model demonstrated the gentamicin loaded SS/PVA hydrogel could effectively inhibit bacterial growth, thus maintain cells viability. This novel hydrogel with antimicrobial activity has shown great potential in wound dressing.
开发一种无毒、高吸水性和抗菌的水凝胶作为皮肤伤口敷料具有重要意义。丝胶蛋白水凝胶是一种理想的敷料材料，因为它具有极好的生物相容性、生物可降解性、保水性、与生物分子的高亲和力、自修复性和促进细胞增殖活性。在这里，我们通过反复冻融将丝胶蛋白（SS）与聚乙烯醇（PVA）混合，制备了 SS/PVA 水凝胶。SS/PVA 水凝胶的光致发光表明 PVA 与丝胶蛋白很好地混合。SS/PVA 水凝胶具有优异的亲水性和溶胀行为，因为其具有多孔结构。PVA 的混合有效地提高了丝胶蛋白的热稳定性，并增强了丝胶蛋白的机械性能，但不影响丝胶蛋白和 PVA 的结晶度。SS/PVA 水凝胶表现出负载和释放小分子药物和银纳米粒子的能力。细胞毒性和免疫毒性试验表明，载有庆大霉素的 SS/PVA 水凝胶对哺乳动物细胞具有极好的细胞相容性。抑菌试验和伤口感染模型表明，载有庆大霉素的 SS/PVA 水凝胶能有效抑制细菌生长，从而保持细胞活力。这种具有抗菌活性的新型水凝胶在伤口敷料方面具有很大的潜力。
