DeBold J F, Malsbury C W
Department of Psychology, Tufts University, Medford, MA 02155.
Physiol Behav. 1989 Oct;46(4):655-60. doi: 10.1016/0031-9384(89)90347-8.
In the first experiment, ovariectomized female hamsters were stereotaxically implanted with bilateral guide cannulae aimed at the medial preoptic area (POA), ventromedial hypothalamus (VMH), or ventral tegmentum (VTA). The following week these females were injected SC with 10 micrograms estradiol benzoate (EB) and then had 27-gauge cannulae containing crystalline progesterone inserted through the guide tubes. Sexual receptivity was observed in 3 of 11 animals with VMH implants of progesterone, in 2 of 10 with VTA progesterone, but in none with POA implants. In the second experiment, the amount of intracranial progesterone was increased by mechanically expelling a 1.5 micrograms progesterone pellet from the tip of each cannula insert. This treatment facilitated receptivity in 10 of 20 hamsters with VTA implants and in 9 of 32 VMH-implanted animals. This induction of receptivity required approximately 2 hr. Progesterone pellets in the POA, mammillary region, and lateral mesencephalon were generally ineffective. In hamsters, progesterone into either the VMH or the VTA is sufficient to facilitate receptivity, although neither site is highly sensitive to progesterone. These results differ from those in recent studies in rats and this difference may reflect important species differences in the control of lordosis.
在第一个实验中，对去卵巢的雌性仓鼠进行立体定位，双侧植入导向套管，目标分别是内侧视前区（POA）、腹内侧下丘脑（VMH）或腹侧被盖区（VTA）。接下来的一周，给这些雌性仓鼠皮下注射10微克苯甲酸雌二醇（EB），然后通过导向管插入含有结晶孕酮的27号套管。在11只植入VMH孕酮的动物中有3只表现出性接受能力，10只植入VTA孕酮的动物中有2只表现出性接受能力，但植入POA孕酮的动物中没有一只表现出性接受能力。在第二个实验中，通过机械方式从每个套管插入端挤出1.5微克孕酮丸粒，增加颅内孕酮的量。这种处理使20只植入VTA的仓鼠中有10只、32只植入VMH的动物中有9只表现出接受能力。这种接受能力的诱导大约需要2小时。在POA、乳头体区域和外侧中脑植入孕酮丸粒通常无效。在仓鼠中，将孕酮注入VMH或VTA足以促进接受能力，尽管这两个部位对孕酮都不是高度敏感。这些结果与最近对大鼠的研究结果不同，这种差异可能反映了在脊柱前凸控制方面重要的物种差异。
