de Oliveira T C, Sogayar R, Salata E
Rev Inst Med Trop Sao Paulo. 1989 Mar-Apr;31(2):95-9. doi: 10.1590/s0036-46651989000200006.
Sera of 20 guinea-pigs experimentally infected with Trypanosoma evansi were obtained in order to compare the efficacy of gel diffusion, indirect immunofluorescence and agglutination tests, to detect antibodies to T. evansi. The fluorescent antibody test was positive in six (6) animals and the antibody titres were very low (1:4 to 1:16). The agglutination test detected trypanosomal antibodies in sera one (1) week after infection. After two (2) weeks all animals were positive with high titres (1:8.000 to 1:250.000). Agglutination was inhibited when sera were treated with 2-Mercapto-ethanol. This fact suggests that IgM is the principal class of antibodies in sera of infected guinea-pigs. Precipitating antibodies were not detected during the course of infection.
为了比较凝胶扩散试验、间接免疫荧光试验和凝集试验检测伊氏锥虫抗体的效果，获取了20只经伊氏锥虫实验感染的豚鼠的血清。荧光抗体试验在6只动物中呈阳性，抗体效价非常低（1:4至1:16）。凝集试验在感染后1周检测到血清中的锥虫抗体。2周后，所有动物均呈阳性，且效价较高（1:8000至1:250000）。当血清用2-巯基乙醇处理时，凝集受到抑制。这一事实表明，IgM是感染豚鼠血清中的主要抗体类别。在感染过程中未检测到沉淀抗体。
