Shen Chen, Xu Yingwu, Huang Jianqin, Wang Zhengjia, Qiu Jiani, Huang Youjun
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Zhejiang 311300, China.
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin'an, Zhejiang 311300, China.
Plant Physiol Biochem. 2014 Oct;83:142-50. doi: 10.1016/j.plaphy.2014.07.020. Epub 2014 Aug 1.
The full ORFs of three floral genes in hickory (Carya cathayensis Sarg.), CcAGL24 (the AGAMOUS-LIKE24 homolog), CcSOC1 (the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 homolog) and CcAP1 (the APETALA1 homolog) are derived using a 5' RACE PCR protocol. Through sequence alignment and phylogenetic analysis, it is demonstrated that the three genes belong to the MADS-Box family. According to the evolutionary trees of the three genes, the homologous genes from the same family cluster well together, while those from different orders doesn't match evolutionary regularity of individual organisms. The result of Quantitative RT-PCR analysis shows that the transcriptional levels of the three genes are up-regulated in early stage and down-regulated in late stage in pistillate floral development. However, it takes different time to reach respective expression peak among the three genes. In staminate floral development, the transcription trend of the three genes is up-regulated, subsequently down-regulated, and then up-regulated again. Nevertheless, those trajectories, peaks, expression levels, inflection points are different in pistillate floral development. The result suggests that their functions are different in between pistillate and staminate floral development. The probable ordinal site of the three genes in the flowering network from top down is CcAGL24, CcSOC1, and CcAP1, which is identical to that in herbaceous plants. Moreover, several adverse environmental factors trigger several negative genes and then confine the development of staminate floral buds. Our results suggest the possible relationship among the three critical floral genes and their functions throughout the floral development in hickory.
利用5' RACE PCR技术获得了山核桃（Carya cathayensis Sarg.）中三个花发育基因的完整开放阅读框，分别为CcAGL24（AGAMOUS-LIKE24同源基因）、CcSOC1（CONSTANS1过表达抑制因子同源基因）和CcAP1（APETALA1同源基因）。通过序列比对和系统发育分析，证明这三个基因属于MADS-Box家族。根据这三个基因的进化树，同一家族的同源基因聚类良好，而不同科的同源基因则不符合个体生物的进化规律。定量RT-PCR分析结果表明，在雌花发育过程中，这三个基因的转录水平在早期上调，后期下调。然而，这三个基因达到各自表达峰值的时间不同。在雄花发育过程中，这三个基因的转录趋势是先上调，随后下调，然后再次上调。然而，在雌花发育过程中，这些轨迹、峰值、表达水平、拐点是不同的。结果表明，它们在雌花和雄花发育中的功能不同。这三个基因在开花网络中从顶部到底部可能的顺序位点是CcAGL24、CcSOC1和CcAP1，这与草本植物中的顺序相同。此外，几种不利的环境因素会触发几个负调控基因，从而限制雄花芽的发育。我们的结果表明了山核桃花发育过程中这三个关键花发育基因之间可能的关系及其功能。
