Wang Zheng Jia, Huang Jian Qin, Huang You Jun, Li Zheng, Zheng Bing Song
Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
Planta. 2012 Aug;236(2):613-21. doi: 10.1007/s00425-012-1634-x. Epub 2012 Apr 6.
Hickory (Carya cathayensis Sarg.) is an economically important woody plant in China, but its long juvenile phase delays yield. MicroRNAs (miRNAs) are critical regulators of genes and important for normal plant development and physiology, including flower development. We used Solexa technology to sequence two small RNA libraries from two floral differentiation stages in hickory to identify miRNAs related to flower development. We identified 39 conserved miRNA sequences from 114 loci belonging to 23 families as well as two novel and ten potential novel miRNAs belonging to nine families. Moreover, 35 conserved miRNAs and two novel miRNAs were detected. Twenty miRNA sequences from 49 loci belonging to 11 families were differentially expressed; all were up-regulated at the later stage of flower development in hickory. Quantitative real-time PCR of 12 conserved miRNA sequences, five novel miRNA families, and two novel miRNA*s validated that all were expressed during hickory flower development, and the expression patterns were similar to those detected with Solexa sequencing. Finally, a total of 146 targets of the novel and conserved miRNAs were predicted. This study identified a diverse set of miRNAs that were closely related to hickory flower development and that could help in plant floral induction.
山核桃（Carya cathayensis Sarg.）是中国一种具有重要经济价值的木本植物，但其较长的幼年期延缓了产量。微小RNA（miRNA）是基因的关键调节因子，对植物正常发育和生理过程（包括花发育）至关重要。我们利用Solexa技术对山核桃两个花分化阶段的两个小RNA文库进行测序，以鉴定与花发育相关的miRNA。我们从属于23个家族的114个位点中鉴定出39个保守的miRNA序列，以及属于9个家族的2个新miRNA和10个潜在新miRNA。此外，还检测到35个保守的miRNA和2个新的miRNA。来自11个家族49个位点的20个miRNA序列差异表达；在山核桃花发育后期均上调。对12个保守miRNA序列、5个新miRNA家族和2个新miRNA*进行定量实时PCR验证，结果表明它们在山核桃花发育过程中均有表达，且表达模式与Solexa测序检测到的相似。最后，预测了新的和保守miRNA的总共146个靶标。本研究鉴定出了一组与山核桃花发育密切相关的多样miRNA，有助于植物的花期诱导。
