Gressler L T, Oliveira C B, Coradini K, Dalla Rosa L, Grando T H, Baldissera M D, Zimmermann C E, Da Silva A S, Almeida T C, Hermes C L, Wolkmer P, Silva C B, Moreira K L S, Beck R C R, Moresco R N, Da Veiga M L, Stefani L M, Monteiro S G
Department of Microbiology and Parasitology,Universidade Federal de Santa Maria (UFSM),Santa Maria,Brazil.
Faculdade de Farmácia, Programa de Pós-Graduação em Ciências Farmacêuticas,Universidade Federal do Rio Grande do Sul (UFRGS),Porto Alegre,Brazil.
Parasitology. 2015 Mar;142(3):439-48. doi: 10.1017/S0031182014001292. Epub 2014 Aug 19.
This study aimed to evaluate in vitro and in vivo trypanocidal activity of free and nanoencapsulated curcumin against Trypanosoma evansi. In vitro efficacy of free curcumin (CURC) and curcumin-loaded in lipid-core nanocapsules (C-LNCs) was evaluated to verify their lethal effect on T. evansi. To perform the in vivo tests, T. evansi-infected animals were treated with CURC (10 and 100 mg kg(-1), intraperitoneally [i.p.]) and C-LNCs (10 mg kg(-1), i.p.) during 6 days, with the results showing that these treatments significantly attenuated the parasitaemia. Infected untreated rats showed protein peroxidation and an increase of nitrites/nitrates, whereas animals treated with curcumin showed a reduction on these variables. As a result, the activity of antioxidant enzymes (superoxide dismutase and catalase) differs between groups (P<0.05). Infected animals and treated with CURC exhibited a reduction in the levels of alanine aminotransferase and creatinine, when compared with the positive control group. The use of curcumin in vitro resulted in a better parasitaemia control, an antioxidant activity and a protective effect on liver and kidney functions of T. evansi-infected adult male Wistar rats.
本研究旨在评估游离型和纳米包封型姜黄素对伊氏锥虫的体外和体内杀锥虫活性。评估了游离姜黄素（CURC）和负载于脂质核纳米胶囊中的姜黄素（C-LNCs）的体外功效，以验证它们对伊氏锥虫的致死作用。为进行体内试验，在6天内对感染伊氏锥虫的动物腹腔注射CURC（10和100mg/kg）和C-LNCs（10mg/kg），结果表明这些治疗显著减轻了寄生虫血症。未治疗的感染大鼠表现出蛋白质过氧化以及亚硝酸盐/硝酸盐增加，而用姜黄素治疗的动物在这些变量上有所降低。结果，各实验组之间抗氧化酶（超氧化物歧化酶和过氧化氢酶）的活性存在差异（P<0.05）。与阳性对照组相比，感染并用CURC治疗的动物丙氨酸转氨酶和肌酐水平降低。体外使用姜黄素可更好地控制寄生虫血症，具有抗氧化活性，并对感染伊氏锥虫的成年雄性Wistar大鼠的肝脏和肾脏功能有保护作用。
