Baldissera Matheus D, Da Silva Aleksandro S, Oliveira Camila B, Santos Roberto C V, Vaucher Rodrigo A, Raffin Renata P, Gomes Patrícia, Dambros Maria G C, Miletti Luiz C, Boligon Aline A, Athayde Margareth L, Monteiro Silvia G
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil; Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
Exp Parasitol. 2014 Jun;141:21-7. doi: 10.1016/j.exppara.2014.03.007. Epub 2014 Mar 19.
This study aimed to evaluate the Trypanosoma evansi susceptibility to tea tree oil (TTO - Melaleuca alternifolia) and tea tree oil nanocapsules (TTO nanocapsules) in vitro and in vivo tests. In vitro, we observed a mortality curve of trypomastigotes proportional to dose, i.e., the TTO and TTO nanocapsules have trypanocidal effect. Treatment with TTO in vivo was assessed in experiments (I and II). For Experiment I, T. evansi infected mice were treated with TTO and/or combinations of essential oil with chemotherapy (diminazene aceturate - D.A.). Treatment with TTO at a dose of 1mLkg(-1) was able to extend animal longevity, but had no curative efficacy. However, when TTO was combined with D.A. a disease curative efficacy of 100% for disease was observed, a much better result than the D.A. treatment (33.3%). In Experiment II, T. evansi infected mice were treated with TTO nanocapsules with doses of 0.3, 0.6 and 0.9mLkg(-1). Animals treated with 0.9mLkg(-1) showed higher longevity however without curative effect. Active compounds present in natural products, such as M. alternifolia, may potentiate the treatment of trypanosomosis when associated with other trypanocidal drugs.
本研究旨在通过体外和体内试验评估伊氏锥虫对茶树油（互叶白千层茶树油 - TTO）和茶树油纳米胶囊（TTO纳米胶囊）的敏感性。在体外，我们观察到锥鞭毛体的死亡率曲线与剂量成正比，即TTO和TTO纳米胶囊具有杀锥虫作用。在实验（I和II）中评估了TTO的体内治疗效果。对于实验I，用TTO和/或精油与化疗药物（乙酰氨基苯脒 - D.A.）的组合治疗感染伊氏锥虫的小鼠。以1mLkg(-1)的剂量使用TTO能够延长动物寿命，但没有治愈效果。然而，当TTO与D.A.联合使用时，观察到疾病治愈率为100%，比D.A.治疗（33.3%）的效果好得多。在实验II中，用剂量为0.3、0.6和0.9mLkg(-1)的TTO纳米胶囊治疗感染伊氏锥虫的小鼠。用0.9mLkg(-1)治疗的动物显示出更长的寿命，但没有治愈效果。天然产物中存在的活性化合物，如互叶白千层，与其他杀锥虫药物联合使用时可能会增强对锥虫病的治疗效果。
