Yan Denglu, Pei Fuxing, Song Yancheng
Nanshan Hospital of Shenzhen University, Shenzhen 518052, People's Republic of China.
J Orthop Surg Res. 2014 Aug 20;9:78. doi: 10.1186/s13018-014-0078-3.
There were no studies on the macrophage colony-stimulating factor (M-CSF), receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG) in the pathogenesis of Kashin-Beck disease (KBD). The objective of the present study was to investigate the serum M-CSF, RANKL and OPG in rats fed with KBD-affected diet.
Ninety Wistar rats were divided into five groups. The rats received standard commercial feed with or without T-2 toxin additive, low protein feed with or without or T-2 toxin additive and the KBD-affected feed. The serum bioactivity of M-CSF, RANKL and OPG was tested by enzyme-linked immunosorbent assay.
The serum levels of M-CSF in E group rats were higher than those in the other groups in the five groups (P < 0.01). The serum levels of RANKL and OPG in E group rats were highest in the five groups and have significant difference compared to the other groups (P < 0.05).
The molecule of M-CSF, RANKL and OPG may be involved in the regulation of epiphyseal plate injury and repair in KBD, and its participation in the pathogenesis of KBD should be studied in the future.
目前尚无关于巨噬细胞集落刺激因子（M-CSF）、核因子κB受体激活蛋白配体（RANKL）和骨保护素（OPG）在大骨节病（KBD）发病机制中的研究。本研究旨在探讨喂食受KBD影响饮食的大鼠血清中的M-CSF、RANKL和OPG。
将90只Wistar大鼠分为五组。大鼠分别接受添加或不添加T-2毒素的标准商业饲料、添加或不添加T-2毒素的低蛋白饲料以及受KBD影响的饲料。采用酶联免疫吸附测定法检测M-CSF、RANKL和OPG的血清生物活性。
五组中，E组大鼠血清M-CSF水平高于其他组（P<0.01）。E组大鼠血清RANKL和OPG水平在五组中最高，与其他组相比有显著差异（P<0.05）。
M-CSF、RANKL和OPG分子可能参与KBD中骺板损伤与修复的调节，其在KBD发病机制中的作用有待进一步研究。
