Anderson R L, Lefever F R, Miller N S, Maurer J K
Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45247.
Food Chem Toxicol. 1989 Dec;27(12):777-9. doi: 10.1016/0278-6915(89)90107-5.
To ascertain whether the bladder mass increase and epithelial hyperplasia induced by 5% dietary sodium saccharin (NaS) in short-term experiments with rats are caused by increased urinary excretion of indican associated with this treatment, the responses of the urine and bladder induced by 1.5% indole (Id) ingestion were compared with those induced by 5% NaS and 1.5% Id + 5% NaS. Id and NaS, when fed alone, produced equivalent increases in bladder mass and both compounds induced epithelial hyperplasia, but Id ingestion was associated with much greater urinary indican excretion (5 mg/g diet ingested) than was NaS (0.3 mg/g diet ingested). When Id and NaS were ingested together, the bladder mass increase was additive, but the epithelial hyperplasia was not exacerbated over that observed with each alone, and the urinary indican was equivalent to that produced by Id alone. These findings suggest that a high level of urinary indican excretion is associated with an increase in bladder mass and epithelial hyperplasia (Id treatment) but indicate that the relatively low urinary indican level obtained by NaS feeding alone is unlikely to be responsible for the bladder responses noted with this compound.
为确定在大鼠短期实验中，5%的膳食糖精钠（NaS）所诱导的膀胱肿块增大和上皮增生是否由该处理导致的尿蓝母排泄增加所致，将摄入1.5%吲哚（Id）所诱导的尿液和膀胱反应与5% NaS及1.5% Id + 5% NaS所诱导的反应进行了比较。单独喂食Id和NaS时，二者使膀胱肿块出现同等程度的增加，且两种化合物均诱导上皮增生，但摄入Id比摄入NaS时尿蓝母排泄量高得多（摄入5 mg/g饮食）（摄入NaS为0.3 mg/g饮食）。当同时摄入Id和NaS时，膀胱肿块的增加具有相加性，但上皮增生并未比单独使用时更严重，且尿蓝母与单独使用Id时产生的量相当。这些发现表明，高水平的尿蓝母排泄与膀胱肿块增加和上皮增生（Id处理）相关，但表明单独喂食NaS所获得的相对较低的尿蓝母水平不太可能是该化合物所引起的膀胱反应的原因。
