一种用于测量培养肝细胞合成的大鼠α1-酸性糖蛋白的酶联免疫测定法。
An enzyme-linked immunoassay for the measurement of rat alpha 1-acid glycoprotein synthesized by cultured hepatocytes.
作者信息
Biou D, Daveau M, Rigal O, Hiron M, Porquet D, Lebreton J P
机构信息
Laboratoire de Biochimie (URA 017), U.E.R. Sciences Pharmaceutiques, Chatenay-Malabry, France.
出版信息
J Immunol Methods. 1989 Dec 20;125(1-2):1-4. doi: 10.1016/0022-1759(89)90070-7.
We have developed a sandwich ELISA to quantify rat alpha 1-acid glycoprotein (AGP). The assay correlated well with RID and the minimum detectable concentration was 1 microgram/l. The assay permits high sensitivity determinations of the rate of synthesis of AGP in vitro. The maximum mean rates observed were 1500 and 1800 ng/24 h/10(6) cells for hepatocytes cultured alone and co-cultured hepatocytes respectively and 39 ng/h/10(6) cells for isolated hepatocytes.
我们开发了一种夹心酶联免疫吸附测定法（ELISA）来定量大鼠α1-酸性糖蛋白（AGP）。该测定法与放射免疫扩散法（RID）相关性良好，最低可检测浓度为1微克/升。该测定法可对体外AGP的合成速率进行高灵敏度测定。单独培养的肝细胞和共培养的肝细胞观察到的最大平均速率分别为1500和1800纳克/24小时/10⁶个细胞，分离的肝细胞为39纳克/小时/10⁶个细胞。
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