Hafner G, Luley C, Prellwitz W
Institut für Klinische Chemie, Johannes Gutenberg-Universität Mainz, FRG.
J Clin Chem Clin Biochem. 1989 Sep;27(9):601-4. doi: 10.1515/cclm.1989.27.9.601.
A fully mechanized immunonephelometric method is described for the rapid and specific determination of apolipoprotein A-II in serum. The method utilizes commercially available sheep antiserum against human apolipoprotein A-II. Nephelometry was performed with the Behring Nephelometer Analyzer (BNA). A single determination can be performed in 12 minutes, requiring 10 microliters sample volume. The measuring range is about 0.08 to 1.25 g/l apolipoprotein A-II. Precision is characterized by intra-assay coefficients of variation of 3.37%, 3.93% and 4.49% for apolipoprotein A-II concentrations of 1.22 g/l, 0.376 g/l and 0.185 g/l, and inter-assay coefficients of variation of 4.27% for an apolipoprotein A-II concentration of 0.404 g/l, respectively. Accuracy of the method is shown by the close correlation of results with those from radial immunodiffusion (r = 0.913, y = 1.091 x -0.033, n = 75).
描述了一种用于快速、特异性测定血清中载脂蛋白A-II的全自动免疫比浊法。该方法使用市售的抗人载脂蛋白A-II羊抗血清。用贝林比浊分析仪（BNA）进行比浊测定。单次测定可在12分钟内完成，所需样品体积为10微升。测量范围约为0.08至1.25 g/l载脂蛋白A-II。精密度的特征在于，载脂蛋白A-II浓度为1.22 g/l、0.376 g/l和0.185 g/l时，批内变异系数分别为3.37%、3.93%和4.49%；载脂蛋白A-II浓度为0.404 g/l时，批间变异系数为4.27%。该方法的准确性通过结果与放射免疫扩散结果的密切相关性得以体现（r = 0.913，y = 1.091x - 0.033，n = 75）。
