通过酶联免疫吸附测定法检测牛感染睡眠嗜组织菌后针对其生物膜胞外多糖的抗体。
Detection of antibodies to the biofilm exopolysaccharide of Histophilus somni following infection in cattle by enzyme-linked immunosorbent assay.
作者信息
Pan Yu, Fisher Taylor, Olk Christina, Inzana Thomas J
机构信息
Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Department of Biomedical Sciences and Pathobiology, Blacksburg, Virginia, USA.
Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Department of Biomedical Sciences and Pathobiology, Blacksburg, Virginia, USA Virginia Tech Carilion School of Medicine, Department of Basic Sciences, Roanoke, Virginia, USA
出版信息
Clin Vaccine Immunol. 2014 Oct;21(10):1463-7. doi: 10.1128/CVI.00384-14. Epub 2014 Aug 20.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibodies to Histophilus somni exopolysaccharide (EPS), which is created during biofilm formation. When an index value of 0.268 was used, the sensitivity of the assay for infected calves was 90.5% at 3 weeks postinfection, but the number of positive animals increased by week 4. The specificity of the assay for healthy calves was 92.5%. The EPS ELISA may aid in identifying calves with H. somni diseases.
摘要
开发了一种酶联免疫吸附测定（ELISA）法来检测牛针对睡眠嗜血杆菌胞外多糖（EPS）的抗体，该胞外多糖是在生物膜形成过程中产生的。当使用0.268的指数值时，该测定法对感染犊牛在感染后3周的敏感性为90.5%，但到第4周时阳性动物数量增加。该测定法对健康犊牛的特异性为92.5%。EPS ELISA可能有助于识别患有睡眠嗜血杆菌病的犊牛。
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