Tai Helen H, Worrall Kraig, Pelletier Yvan, De Koeyer David, Calhoun Larry A
Agriculture and Agri-Food Canada Potato Research Centre, P.O. Box 20280, 850 Lincoln Road, Fredericton, New Brunswick, Canada E3B 4Z7.
J Agric Food Chem. 2014 Sep 10;62(36):9043-55. doi: 10.1021/jf502508y. Epub 2014 Aug 27.
The Colorado potato beetle Leptinotarsa decemlineata (Say) (CPB) is a coleopteran herbivore that feeds on the foliage on Solanum species, in particular, potato. Six resistant wild Solanum species were identified, and two of these species had low levels of glycoalkaloids. Comparative analysis of the untargeted metabolite profiles of the foliage using UPLC-qTOF-MS was done to find metabolites shared between the wild species but not with Solanum tuberosum (L.) to identify resistance-related metabolites. It was found that only S. tuberosum produced the triose glycoalkaloids solanine and chaconine. Instead, the six wild species produced glycoalkaloids that shared in common tetrose sugar side chains. Additionally, there were non-glycoalkaloid metabolites associated with resistance including hydroxycoumarin and a phenylpropanoid, which were produced in all wild species but not in S. tuberosum.
科罗拉多马铃薯甲虫Leptinotarsa decemlineata (Say)（CPB）是一种鞘翅目食草动物，以茄属植物的叶子为食，尤其是马铃薯。已鉴定出六种具有抗性的野生茄属植物，其中两种植物的糖苷生物碱含量较低。利用超高效液相色谱-四极杆飞行时间质谱（UPLC-qTOF-MS）对叶片的非靶向代谢物谱进行比较分析，以寻找野生种之间共有的但马铃薯（Solanum tuberosum (L.)）没有的代谢物，从而鉴定与抗性相关的代谢物。结果发现，只有马铃薯产生了丙糖糖苷生物碱茄碱和查茄碱。相反，这六种野生种产生的糖苷生物碱具有共同的丁糖糖侧链。此外，还有与抗性相关的非糖苷生物碱代谢物，包括羟基香豆素和一种苯丙烷类化合物，它们在所有野生种中都有产生，但在马铃薯中没有。
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