Takahashi Mitsuko, Sakurai Katsumi, Fujii Hisashi, Saito Koichi
J AOAC Int. 2014 Jul-Aug;97(4):1195-201. doi: 10.5740/jaoacint.13-038.
Components that could be used as indicators for the discrimination of senna (Cassia angustifolia) from other cassia plants contained in health teas were identified, and an analytical method for the components was developed. Our results revealed two components in senna that were not found in other Cassia spp. widely used in health teas, such as C. alata, C. corymbosa, C. obtusifolia, and C. occidentalis. Structural elucidation of the two components showed that they were isorhamnetin-3-O-gentiobioside and tinnevellin glucoside. We analyzed commercial health teas using the HPLC method developed in this study. The two indicator components were detected at 366 nm using an RP C18 column and gradient elution with a mixture of water and acetonitrile (with formic acid), as the mobile phase. Our analytical method by HPLC enabled the differentiation of senna from other Cassia plants present in health teas in which sennosides A and B were detected. Moreover, this method allowed us to predict the parts of senna in health teas from the amounts of isorhamnetin-3-O-gentiobioside and tinnevellin glucoside contained in the teas.
确定了可作为区分番泻叶（狭叶番泻）与保健茶中其他决明属植物的指标的成分，并开发了这些成分的分析方法。我们的结果显示，番泻叶中有两种成分在其他广泛用于保健茶的决明属植物中未被发现，如翅荚决明、伞房决明、钝叶决明和望江南。对这两种成分的结构解析表明，它们是异鼠李素-3-O-龙胆二糖苷和亭奈维林葡萄糖苷。我们使用本研究中开发的高效液相色谱法（HPLC）分析了市售保健茶。使用反相C18柱，以水和乙腈（含甲酸）的混合物作为流动相进行梯度洗脱，在366nm处检测到这两种指示成分。我们的HPLC分析方法能够区分番泻叶与保健茶中存在的其他决明属植物，这些保健茶中检测到了番泻苷A和B。此外，该方法使我们能够根据茶中所含异鼠李素-3-O-龙胆二糖苷和亭奈维林葡萄糖苷的量来预测保健茶中番泻叶的含量。
