Wu Qiu-Ping, Wang Zhu-Ju, Tang Li-Ying, Fu Mei-Hong, He Yan, Fang Jing, Gong Qian-Feng
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2008 Feb;33(4):363-5.
To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.
The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.
The results showed that 5 effective components all separated well and showed good linearity.
The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.
建立一种测定狭叶番泻叶中主要化学成分含量的方法。
采用Diamonsil C18（4.6 mm×250 mm，5μm）色谱柱，以乙腈-1%乙酸（10:90 - 15:85 - 18:82 - 20:80 - 25:75）梯度洗脱为流动相，流速为1 mL·min⁻¹，柱温40℃，检测波长270 nm。
结果表明5种有效成分均分离良好，线性关系良好。
该方法快速、灵敏、准确、可靠、重复性好，可用于番泻叶的质量控制。
