Widdicombe J H, Ueki I F, Emery D, Margolskee D, Yergey J, Nadel J A
Cardiovascular Research Institute, University of California, San Francisco 94143.
Am J Physiol. 1989 Dec;257(6 Pt 1):L361-5. doi: 10.1152/ajplung.1989.257.6.L361.
Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF2 alpha, 6-keto-PGF1 alpha, and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), methacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine, and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.
采用放射免疫分析法测定犬或人气管上皮原代培养物中环氧化酶产物的释放量。在这两个物种中，缓激肽、血小板活化因子（PAF）和A23187（一种钙离子载体）可使前列腺素（PG）E2的释放速率增加，而使PGF2α、6-酮-PGF1α和血栓素B2的释放量有较小增加。异丙肾上腺素、血管活性肠肽（VIP）、乙酰甲胆碱以及白三烯C4和D4对这些环氧化酶产物的释放没有影响。气相色谱-质谱分析表明，A23187从犬细胞释放的PGE2与PGD2的比例为30:1。缓激肽、A23187、PAF、VIP、乙酰甲胆碱和异丙肾上腺素可刺激犬细胞的短路电流。只有对缓激肽和A23187的反应可通过吲哚美辛预处理而降低。
