Zhuang Jiawei, Shan Zhonggui, Ma Teng, Zhou Xiaobiao, Qiu Shuiwei
Department of Cardiac Surgery, First Affliated Hospital,Xiamen University, Xiamen 361003, China.
Department of Cardiac Surgery, First Affliated Hospital,Xiamen University, Xiamen 361003, China. Email:
Zhonghua Yi Xue Za Zhi. 2014 May 27;94(20):1584-6.
To observe the effects of chemokines CXCL9 and CXCL10 on cardiac allograft acute rejection mediated by alloreactive memory T cells in a retransplantation model.
Heart transplantation was performed 6 weeks after skin grafting. The mice were divided into 3 groups of control (direct heterotopic heart transplantation without skin grafting); experimental (heart transplantation after skin grafting) and syngraft (C57BL/6→C57BL/6, heterotopic heart transplantation) (n = 12 each). Graft survival and the pathological changes of cardiac graft were observed. And related gene expression in cardiac grafts and serum concentration of CXCL9/CXCL10 were detected.
The mean survival time of control and experimental groups was 7.75 and 3.25 days respectively (P < 0.01).Serum concentrations of CXCL9 and CXCL10 in recipient mice were higher in the experimental group than those in the control group. Compared with the control group, the relative gene expressions of CXCL9 and CXCL10 were higher in the experimental group. According to pathological examinations, the histological rank of cardiac allograft was Grade 2.27 ± 0.25 in the control group versus Grade 4.12 ± 0.03 in the experimental group (P < 0.01).
CXCL9 and CXCL10 play critical roles in retransplantation mediated by alloreactive memory T cells. And acute rejection of cardiac allograft is more extensive in retransplantation.
在再次移植模型中观察趋化因子CXCL9和CXCL10对同种异体反应性记忆T细胞介导的心脏移植急性排斥反应的影响。
皮肤移植6周后进行心脏移植。将小鼠分为3组：对照组（直接异位心脏移植，未进行皮肤移植）；实验组（皮肤移植后进行心脏移植）和同基因移植组（C57BL/6→C57BL/6，异位心脏移植）（每组n = 12）。观察移植物存活情况及心脏移植物的病理变化。检测心脏移植物中相关基因表达及血清CXCL9/CXCL10浓度。
对照组和实验组的平均存活时间分别为7.75天和3.25天（P < 0.01）。实验组受体小鼠血清CXCL9和CXCL10浓度高于对照组。与对照组相比，实验组CXCL9和CXCL10的相对基因表达更高。病理检查显示，心脏同种异体移植物的组织学分级在对照组为2.27±0.25级，在实验组为4.12±0.03级（P < 0.01）。
CXCL9和CXCL10在同种异体反应性记忆T细胞介导的再次移植中起关键作用。并且心脏同种异体移植物的急性排斥反应在再次移植中更广泛。
