Wang Wenjuan, Wang Zhiyuan, Bai Wenpei, Zhang Hui, Ma Xiaoyan, Yang Mukun, Wang Ke, Zhu Sainan, Jia Jing, Qin Lihua
Anatomy and Embryology Department, Peking University Health Science Center, No. 38 Xueyuan Road, Haidian District, Beijing, China.
Obstetrics and Gynecology Department, Peking University First Hospital, No. 8 Xishiku Street, Xicheng District, Beijing, China.
Acta Histochem. 2014 Oct;116(8):1259-69. doi: 10.1016/j.acthis.2014.07.010. Epub 2014 Aug 18.
The purpose of this study was to investigate the difference in neuronal activity in the preoptic area of the hypothalamus (POAH) under low estrogen condition induced by ovariectomy. One hundred and twenty sham-operated (SHAM) and ovariectomized (OVX) rats were placed in different temperatures for 2h. Twelve rats from each group were stimulated by 4°C, 10°C, 25°C, 33°C and 38°C, respectively. c-Fos expression in the POAH was detected by immunohistochemistry. Following exposure to warm and cold stimuli, there were markedly lower c-Fos-positive cell densities in the OVX group compared with the SHAM group in the median preoptic nucleus (MnPO) at 4°C, 10°C, 33°C and 38°C, in the medial preoptic area (MPA) at 25°C and 38°C, in the ventromedial preoptic nucleus (VMPO) at 4°C, 10°C and 38°C and in the ventrolateral preoptic nucleus (VLPO) at 4°C and 38°C. Both temperature and surgery had an impact on c-Fos expression by two-way ANOVA method except in the lateral preoptic area (LPO). c-Fos expression differed within different nuclei of the two groups in the same and different temperature stimuli. This indicated that the temperature-sensitive nuclei in the POAH exhibited lower and different activities during temperature stimuli following ovariectomy, which possibly resulted in abnormal thermoregulation and menopausal symptoms.
本研究的目的是探讨卵巢切除诱导的低雌激素状态下，下丘脑视前区（POAH）神经元活动的差异。120只假手术（SHAM）和去卵巢（OVX）大鼠被置于不同温度环境中2小时。每组分别有12只大鼠接受4°C、10°C、25°C、33°C和38°C的刺激。采用免疫组织化学法检测POAH中c-Fos的表达。在暴露于温暖和寒冷刺激后，在4°C、10°C、33°C和38°C时，OVX组视前正中核（MnPO）中的c-Fos阳性细胞密度明显低于SHAM组；在25°C和38°C时，内侧视前区（MPA）中的c-Fos阳性细胞密度明显低于SHAM组；在4°C、10°C和38°C时，腹内侧视前核（VMPO）中的c-Fos阳性细胞密度明显低于SHAM组；在4°C和38°C时，腹外侧视前核（VLPO）中的c-Fos阳性细胞密度明显低于SHAM组。除外侧视前区（LPO）外，温度和手术均通过双向方差分析方法对c-Fos表达产生影响。在相同和不同温度刺激下，两组不同核团中的c-Fos表达存在差异。这表明，去卵巢后，POAH中的温度敏感核团在温度刺激期间表现出较低且不同的活性，这可能导致体温调节异常和绝经症状。
