Parillo Francesco, Maranesi Margherita, Mignini Fiorenzo, Marinelli Lisa, Di Stefano Antonio, Boiti Cristiano, Zerani Massimo
Scuola di Bioscienze e Medicina veterinaria, Università di Camerino, Matelica, Italy.
Dipartimento di Scienze biopatologiche veterinarie ed Igiene delle produzioni animali e alimentari, Sezione di Fisiologia, Università di Perugia, Perugia, Italy.
PLoS One. 2014 Aug 22;9(8):e104797. doi: 10.1371/journal.pone.0104797. eCollection 2014.
Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well as the in vitro effects of DA on hormone productions by CL in pseudopregnant rabbits. Immunoreactivity and gene expression for D1R decreased while that for D3R increased in luteal and blood vessel cells from early to late pseudopregnant stages. DA immunopositivity was evidenced only in luteal cells. The DA and D1R agonist increased in vitro release of progesterone and prostaglandin E2 (PGE2) by early CL, whereas the DA and D3R agonist decreased progesterone and increased PGF2α in vitro release by mid- and late CL. These results provide evidence that the DA/DR system exerts a dual modulatory function in the lifespan of CL: the DA/D1R is luteotropic while the DA/D3R is luteolytic. The present data shed new light on the physiological mechanisms regulating luteal activity that might improve our ability to optimize reproductive efficiency in mammal species, including humans.
已发现1型多巴胺（DA）受体（DR）（D1R）在多种物种的黄体细胞中表达，但DA/DRs系统对黄体（CL）功能的内在作用仍不清楚。本实验旨在研究假孕兔黄体中DR类型的表达、DA的存在情况，以及DA对黄体激素分泌的体外影响。从假孕早期到晚期，黄体和血管细胞中D1R的免疫反应性和基因表达降低，而D3R的免疫反应性和基因表达增加。仅在黄体细胞中检测到DA免疫阳性。DA和D1R激动剂可增加早期黄体体外孕酮和前列腺素E2（PGE2）的释放，而DA和D3R激动剂可减少中期和晚期黄体体外孕酮的释放，并增加PGF2α的释放。这些结果表明，DA/DR系统在黄体寿命中发挥双重调节作用：DA/D1R具有促黄体作用，而DA/D3R具有溶黄体作用。本研究结果为调节黄体活性的生理机制提供了新的见解，可能有助于提高包括人类在内的哺乳动物的生殖效率。
