Li Yansong, Lu Shiying, Liu Zengshan, Sun Lihui, Guo Junfei, Hu Pan, Zhang Junhui, Zhang Yuanyuan, Wang Yang, Ren Honglin, Meng Xingyu, Zhou Yu
Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, PR China.
Jilin Bussiness and Technology College, Jilin University, Changchun 130062, PR China.
Food Chem. 2015 Jan 15;167:40-4. doi: 10.1016/j.foodchem.2014.06.085. Epub 2014 Jul 1.
Phenylethanolamine A (PEA) is a phenethanolamine member of the family of β-adrenergic agonists compounds illegally used as feed additives for growth promotion. In this study, PEA was covalently linked to carrier protein cationized bovine serum albumin (cBSA) and egg albumin (OVA). A monoclonal antibody specific for PEA was produced and characteristics of monoclonal antibody (McAb) were studied. Based on the McAb, an enzyme-linked immunosorbent assay (ELISA) for detection of PEA was developed, which showed an IC50 value of 6.25 ng mL(-1) and a detection limit of 0.19 ng mL(-1). The average recovery of PEA from spiked samples was 103.4%, which demonstrated that the matrices of meat where PEA may be found do not interfere with the assay.
苯乙醇胺A（PEA）是β-肾上腺素能激动剂化合物家族中的一种苯乙醇胺，被非法用作促进生长的饲料添加剂。在本研究中，PEA与载体蛋白阳离子化牛血清白蛋白（cBSA）和卵清蛋白（OVA）共价连接。制备了一种针对PEA的单克隆抗体，并研究了单克隆抗体（McAb）的特性。基于该单克隆抗体，开发了一种用于检测PEA的酶联免疫吸附测定（ELISA）方法，其IC50值为6.25 ng mL⁻¹，检测限为0.19 ng mL⁻¹。加标样品中PEA的平均回收率为103.4%，这表明可能发现PEA的肉类基质不会干扰该测定。
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