Junhua Li, Chunsheng Li, Meng Wu, Yan Zhang, Xiaofei Ma, Hua Cheng, Jinghui Yan
Biology Inst. of Hebei Academy of Sciences, Shijiazhuang, Hebei, 050051, PR, China.
J Food Sci. 2015 Apr;80(4):T894-9. doi: 10.1111/1750-3841.12814. Epub 2015 Mar 5.
In this study a one-step immunochromatographic assay based on competitive format was developed for the rapid detection of phenylethanolamine A (PEAA) residues in urine and pork samples. A monoclonal antibody against PEAA was produced from BALB/c mice immunized with the PEAA-BSA conjugate. The results of this qualitative test strip were to be interpreted visually. The visual detection limit (VDL) and threshold level of the optimized immunochromatographic assay for PEAA were 0.1 ng/mL and 0.5 ng/mL, respectively. Cross-reactions with other β-agonists were not significant inhibitions to the performance of the test strip assay. The results from the test strip were in a good agreement with those obtained using a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay. The immunochromatographic assay developed here was a useful on-site screening tool that is rapid to use, low in cost, and extremely convenient for the detection of PEAA in urine samples and pork samples.
在本研究中，开发了一种基于竞争模式的一步式免疫层析测定法，用于快速检测尿液和猪肉样品中的苯乙醇胺A（PEAA）残留。用PEAA-BSA偶联物免疫BALB/c小鼠，制备了抗PEAA的单克隆抗体。该定性试纸条的结果通过目视判读。优化后的PEAA免疫层析测定法的目视检测限（VDL）和阈值水平分别为0.1 ng/mL和0.5 ng/mL。与其他β-激动剂的交叉反应对试纸条测定性能无显著抑制作用。试纸条的检测结果与高效液相色谱-串联质谱（HPLC-MS/MS）测定法的结果高度一致。这里开发的免疫层析测定法是一种有用的现场筛查工具，使用快速、成本低，并且非常便于检测尿液样品和猪肉样品中的PEAA。
