Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Products Development Department, Beijing WDWK Biotechnology Co. Ltd, Beijing, PR China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Apr;38(4):586-593. doi: 10.1080/19440049.2021.1877835. Epub 2021 Feb 17.
Brombuterol, a new β-adrenergic agonist to enhance animal growth and increase feeding efficiency, is forbidden as an additive in animal feed for livestock production due to its adverse effects on consumers. In this study, a highly specific and sensitive monoclonal antibody was obtained and an indirect competitive monoclonal ELISA was developed to detect brombuterol, with an IC value of 0.1 μg/kg (μg/L) and no cross-reactivity to other structurally related β-adrenergic agonists. The average recovery of brombuterol using the icELISA method ranged from 72.9% to 106.4% with the coefficient of variation lower than 18.9%, which was determined by analysing spiked animal feed, swine urine, pork and liver samples (n = 5). Finally, the icELISA gave results having a good correlation with those obtained by liquid chromatography-tandem mass spectrometry. These results demonstrated that the developed icELISA for the detection of brombuterol is highly specific, sensitive, and reliable, indicating good potential for use in the area of food safety to improve consumer protection.
溴布特罗是一种新型的β-肾上腺素能激动剂，可提高动物生长速度和饲料效率，由于其对消费者的不良影响，已被禁止作为动物饲料添加剂用于畜牧业生产。本研究获得了一种高特异性和高灵敏度的单克隆抗体，并建立了间接竞争单克隆 ELISA 检测方法，检测溴布特罗的 IC50 值为 0.1μg/kg（μg/L），与其他结构相关的β-肾上腺素能激动剂无交叉反应。通过分析添加动物饲料、猪尿、猪肉和猪肝样品（n=5），icELISA 法测定溴布特罗的平均回收率为 72.9%~106.4%，变异系数低于 18.9%。最后，icELISA 法与液相色谱-串联质谱法的检测结果具有良好的相关性。这些结果表明，所建立的检测溴布特罗的 icELISA 方法具有高度的特异性、灵敏度和可靠性，有望在食品安全领域得到应用，提高消费者的保护水平。
