Faleo Gaetano, Berggren Per-Olof, Pileggi Antonello
Transplantation Research Laboratory, Department of Surgery, University of California, 513 Parnassus Ave., HSE-520, San Francisco, CA, 94143, USA,
Methods Mol Biol. 2014;1186:121-9. doi: 10.1007/978-1-4939-1158-5_9.
Intravital imaging approaches are proving to be essential to address new questions to better understand how the immune system operates. These approaches are especially valuable to characterize the complex organization of immune responses in vivo. Here, we examine how to take advantage of the cornea as a natural body window to apply noninvasive imaging techniques to assess cytotoxic T lymphocyte involvement in the immune rejection process in a model of intraocular allogeneic islet transplantation.
活体成像方法已被证明对于解决新问题以更好地理解免疫系统如何运作至关重要。这些方法对于在体内表征免疫反应的复杂组织尤其有价值。在这里,我们研究如何利用角膜作为天然的身体窗口,应用非侵入性成像技术来评估细胞毒性T淋巴细胞在眼内同种异体胰岛移植模型的免疫排斥过程中的参与情况。