从小鼠干细胞祖细胞生成肥大细胞。

Generation of mast cells from murine stem cell progenitors.

作者信息

Swindle Emily J

机构信息

Faculty of Medicine, Academic Unit of Clinical and Experimental Sciences, Southampton General Hospital, University of Southampton, MPT 810, Rm LF73, SAB, Level F, Tremona Road, Southampton, SO16 6YD, UK,

出版信息

Methods Mol Biol. 2014;1192:63-7. doi: 10.1007/978-1-4939-1173-8_5.

DOI:10.1007/978-1-4939-1173-8_5

PMID:25149484

Abstract

Mouse bone marrow-derived mast cells (mBMMCs) are an invaluable tool for the study of mast cell function from wild-type, knockout, and transgenic mice. This method describes the isolation of mast cell progenitors from the bone marrow of mouse femurs and their subsequent culture in an IL-3-rich culture medium. After 4 weeks, mBMMCs are obtained in high number and are of high purity. Assessment of their granularity by toluidine staining and IgE receptor expression by flow cytometry are also described. These cells are a useful tool in the determination of mast cell function in innate and adaptive immunity.

摘要

小鼠骨髓来源的肥大细胞（mBMMCs）是研究野生型、基因敲除型和转基因小鼠肥大细胞功能的宝贵工具。本方法描述了从小鼠股骨骨髓中分离肥大细胞祖细胞，并随后在富含白细胞介素-3的培养基中进行培养的过程。4周后，可获得大量且纯度高的mBMMCs。还介绍了通过甲苯胺染色评估其颗粒度以及通过流式细胞术检测IgE受体表达的方法。这些细胞是确定肥大细胞在固有免疫和适应性免疫中功能的有用工具。