Kennedy M A, Morris C M, Hollings P E, Fitzgerald P H
Cytogenetic and Molecular Oncology Unit, Christchurch Hospital, New Zealand.
Cytogenet Cell Genet. 1989;52(1-2):50-6. doi: 10.1159/000132838.
The breakpoints of a translocation, t(2;14)(p11;q32), detected in an Epstein-Barr virus-transformed lymphoid B-cell line were mapped by Southern analysis, field-inversion gel electrophoresis, and in situ hybridisation. The translocation involved the immunoglobulin light-chain (kappa) locus on chromosome 2 and the heavy-chain locus on chromosome 14. The breakpoint on chromosome 2 was between VK and CK, most likely within JK. The chromosome 14 break was located within the VH cluster, no more than 220 kb 5' of the productively rearranged JH locus. The translocation probably resulted from an aberrant rearrangement of the kappa light-chain genes.
在一株爱泼斯坦-巴尔病毒转化的淋巴B细胞系中检测到的易位t(2;14)(p11;q32)的断点，通过Southern分析、脉冲场凝胶电泳和原位杂交进行了定位。该易位涉及2号染色体上的免疫球蛋白轻链（κ）基因座和14号染色体上的重链基因座。2号染色体上的断点位于VK和CK之间，很可能在JK内。14号染色体的断点位于VH簇内，在有效重排的JH基因座5'端不超过220 kb处。该易位可能是κ轻链基因异常重排的结果。
