International Centre of Biodynamics, Intrarea Portocalelor 1B, Bucharest 060101, Romania.
National Institule for Laser, Plasma and Radiation Physics, Atomistilor 409, Magurele 077125, Romania.
Biosens Bioelectron. 2015 Jan 15;63:525-532. doi: 10.1016/j.bios.2014.08.004. Epub 2014 Aug 8.
We present novel solutions to surpass current analytic limitations of Magneto-Optical Surface Plasmon Resonance (MOSPR) assays, concerning both the chip structure and the method for data analysis. The structure of the chip is modified to contain a thin layer of Co-Au alloy instead of successive layers of homogeneous metals, as currently used. This alloy presents improved plasmonic and magnetic properties, yet a structural stability similar to Au-SPR chips, allowing for bioaffinity assays in saline solutions. Analyzing the whole reflectivity curve at multiple angles of incidence instead of the reflectivity value at a single incidence angle provides a high signal-to-noise ratio suitable for detection of minute analyte concentrations. Based on assessment of solutions with known refractive indices as well as of a model biomolecular interaction (i.e. IgG-AntiIgG) we demonstrate that the proposed structure of the MOSPR sensing chip and the procedure of data analysis allows for long-time assessment in liquid media with increased sensitivity over standard SPR analyses.
我们提出了新颖的解决方案，以克服当前磁光表面等离子体共振（MOSPR）分析在芯片结构和数据分析方法方面的局限性。我们对芯片结构进行了修改，用一层薄薄的 Co-Au 合金代替了目前使用的连续多层均质金属。这种合金具有改进的等离子体和磁性，但与 Au-SPR 芯片的结构稳定性相似，允许在盐溶液中进行生物亲和性分析。在多个入射角下分析整个反射率曲线而不是在单个入射角下分析反射率值，可以提供高信噪比，适合检测微量分析物浓度。通过评估具有已知折射率的溶液以及模型生物分子相互作用（即 IgG-AntiIgG），我们证明了所提出的 MOSPR 传感芯片结构和数据分析程序允许在液体介质中进行长时间评估，并且比标准 SPR 分析具有更高的灵敏度。
