Ducrocq C, Servy C, Lenfant M
Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique (C.N.R.S.), Gif sur Yvette, France.
FEMS Microbiol Lett. 1989 Nov;53(1-2):219-22. doi: 10.1016/0378-1097(89)90394-7.
Glyceryl trinitrate (GTN) 2 mM was quantitatively converted into its 1 and 2 mononitrate derivatives by Geotrichum candidum, with consumption of the nitrite ions produced. The conversion proceeded at a rate independent of the addition of either organic carbon or organic nitrogen sources. Eight batches of nitrate ester, which were added every 24 hours, were successfully converted as far as during the bioconversion process GTN concentration did not exceed 2 mM. When those limiting conditions were not observed, dramatic toxicity of GTN was noticed.
2 mM的甘油三硝酸酯（GTN）被白色念珠菌定量转化为其单硝酸酯衍生物1和2，同时消耗产生的亚硝酸根离子。该转化过程的速率与有机碳源或有机氮源的添加无关。每24小时添加一批共八批硝酸酯，在生物转化过程中GTN浓度不超过2 mM的情况下成功实现了转化。当未观察到这些限制条件时，GTN会表现出显著的毒性。
