Accashian J V, Vinopal R T, Kim B J, Smets B F
Environmental Engineering Program, Department of Civil and Environmental Engineering, University of Connecticut, Storrs, Connecticut 06269-2037, USA.
Appl Environ Microbiol. 1998 Sep;64(9):3300-4. doi: 10.1128/AEM.64.9.3300-3304.1998.
Nitroglycerin (glycerol trinitrate [GTN]), an explosive and vasodilatory compound, was metabolized by mixed microbial cultures from aeration tank sludge previously exposed to GTN. Aerobic enrichment cultures removed GTN rapidly in the absence of a supplemental carbon source. Complete denitration of GTN, provided as the sole C and N source, was observed in aerobic batch cultures and proceeded stepwise via the dinitrate and mononitrate isomers, with successive steps occurring at lower rates. The denitration of all glycerol nitrate esters was found to be concomitant, and 1, 2-glycerol dinitrate (1,2-GDN) and 2-glycerol mononitrate (2-GMN) were the primary GDN and GMN isomers observed. Denitration of GTN resulted in release of primarily nitrite-N, indicating a reductive denitration mechanism. Biomass growth at the expense of GTN was verified by optical density and plate count measurements. The kinetics of GTN biotransformation were 10-fold faster than reported for complete GTN denitration under anaerobic conditions. A maximum specific growth rate of 0.048 +/- 0.005 h-1 (mean +/- standard deviation) was estimated for the mixed culture at 25 degreesC. Evidence of GTN toxicity was observed at GTN concentrations above 0. 3 mM. To our knowledge, this is the first report of complete denitration of GTN used as a primary growth substrate by a bacterial culture under aerobic conditions.
硝酸甘油（甘油三硝酸酯[GTN]）是一种具有爆炸性和血管舒张作用的化合物，可被先前暴露于GTN的曝气池污泥中的混合微生物培养物代谢。在没有补充碳源的情况下，好氧富集培养物能快速去除GTN。在好氧分批培养中观察到，以GTN作为唯一的碳源和氮源时，GTN能完全脱硝，并通过二硝酸盐和单硝酸盐异构体逐步进行，后续步骤的反应速率较低。发现所有硝酸甘油酯的脱硝过程是同时发生的，并且观察到1,2 - 甘油二硝酸酯（1,2 - GDN）和2 - 甘油单硝酸酯（2 - GMN）是主要的GDN和GMN异构体。GTN的脱硝主要导致亚硝酸盐 - N的释放，表明存在还原脱硝机制。通过光密度和平板计数测量验证了以GTN为代价的生物量增长。GTN生物转化的动力学比厌氧条件下完全GTN脱硝的报道快10倍。在25℃下，混合培养物的最大比生长速率估计为0.048±0.005 h⁻¹（平均值±标准差）。在GTN浓度高于0.3 mM时观察到GTN毒性的证据。据我们所知，这是关于细菌培养物在好氧条件下将GTN用作主要生长底物进行完全脱硝的首次报道。
