使用髓鞘反应性CD4 + T细胞诱导被动性实验性自身免疫性脑脊髓炎
Induction of passive EAE using myelin-reactive CD4+ T cells.
作者信息
McPherson Rhoanne C, Cambrook Helen E, O'Connor Richard A, Anderton Stephen M
机构信息
MRC Centre for Inflammation Research and Centre for Multiple Sclerosis Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
出版信息
Methods Mol Biol. 2014;1193:187-98. doi: 10.1007/978-1-4939-1212-4_17.
Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease.This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.
实验性自身免疫性脑脊髓炎（EAE）是一种中枢神经系统（CNS）的自身免疫性疾病，常被用作多发性硬化症早期炎症阶段的模型，也作为器官特异性自身免疫性疾病的模型。本方案描述了在小鼠中诱导被动性EAE的方法，既可以使用从用髓鞘抗原致敏的小鼠中分离的T细胞，也可以通过使用在髓鞘衍生抗原存在下体外激活的幼稚TCR转基因T细胞来实现。
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