McPherson Rhoanne C, Cambrook Helen E, O'Connor Richard A, Anderton Stephen M
MRC Centre for Inflammation Research and Centre for Multiple Sclerosis Research, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK.
Methods Mol Biol. 2014;1193:187-98. doi: 10.1007/978-1-4939-1212-4_17.
Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease.This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.
实验性自身免疫性脑脊髓炎（EAE）是一种中枢神经系统（CNS）的自身免疫性疾病，常被用作多发性硬化症早期炎症阶段的模型，也作为器官特异性自身免疫性疾病的模型。本方案描述了在小鼠中诱导被动性EAE的方法，既可以使用从用髓鞘抗原致敏的小鼠中分离的T细胞，也可以通过使用在髓鞘衍生抗原存在下体外激活的幼稚TCR转基因T细胞来实现。
