Shi Huijun, Fu Qiang, Ren Yan, Wang Dawei, Qiao Jun, Wang Pengyan, Zhang Hui, Chen Chuangfu
a College of Animal Science and Technology, Shihezi University , Shihezi , Xinjiang , China.
Anim Biotechnol. 2015;26(1):73-9. doi: 10.1080/10495398.2014.902850.
Mx1 protein is I type interferons (IFNs)-induced 76-kDa guanosine triphosphatases (GTPases) that belong to the dynamin superfamily of large GTPases. Mx1 proteins have attracted attention because some display antiviral activity against pathogenic RNA and DNA viruses. Meanwhile, Mx1 gene generally exists in organisms or cells of mammalian, fish and chicken. Blocking a wide range of RNA virus replication by inhibiting nuclear viral mRNA synthesis is a unique property of Mx1 protein. In order to investigate a novel prevention measure against foot-and-mouth disease virus (FMDV) and bovine viral diarrhea virus (BVDV), which frequently break out in Xinjiang Uygur Autonomous Region of China, we investigated the effects of porcine Mx1 protein on FMDV and BVDV replication by measuring viral reverse transcriptase activity at various time intervals. In our study, Mx1 protein was overexpressed in BHK-21 and MDBK cells mediated by lentivirus prior to infect with FMDV and BVDV. FMDV and BVDV replication levels were monitored by quantitative real-Time PCR. The results showed porcine Mx1 overexpression significantly inhibited both FMDV and BVDV replication within 12 and 36 hours post-infection (pi). The finding may provide a new therapeutic approach for preventing from FDMV and BVDV infection.
Mx1蛋白是I型干扰素（IFN）诱导的76 kDa鸟苷三磷酸酶（GTPase），属于大型GTPase的发动蛋白超家族。Mx1蛋白引起了人们的关注，因为有些Mx1蛋白对致病性RNA和DNA病毒具有抗病毒活性。同时，Mx1基因普遍存在于哺乳动物、鱼类和鸡的生物体或细胞中。通过抑制核病毒mRNA合成来阻断多种RNA病毒复制是Mx1蛋白的独特特性。为了研究针对在中国新疆维吾尔自治区频繁爆发的口蹄疫病毒（FMDV）和牛病毒性腹泻病毒（BVDV）的新型预防措施，我们通过在不同时间间隔测量病毒逆转录酶活性，研究了猪Mx1蛋白对FMDV和BVDV复制的影响。在我们的研究中，在感染FMDV和BVDV之前，通过慢病毒介导在BHK-21和MDBK细胞中过表达Mx1蛋白。通过定量实时PCR监测FMDV和BVDV的复制水平。结果显示，猪Mx1过表达在感染后12小时和36小时内显著抑制了FMDV和BVDV的复制。这一发现可能为预防FDMV和BVDV感染提供一种新的治疗方法。
