Molina Inmaculada, Lázaro-Ibáñez Elisa, Pertusa Jose, Debón Ana, Martínez-Sanchís Juan Vicente, Pellicer Antonio
Unidad de Reproducción Humana, Hospital Universitari i Politècnic La Fe de Valencia, Avenida Campanar 21, 46009 Valencia, Spain;; Departamento de Ciencia Animal, Universitat Politécnica de València, 46022 Valencia, Spain;
Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, 00014 Helsinki, Finland;
Reprod Biomed Online. 2014 Oct;29(4):470-80. doi: 10.1016/j.rbmo.2014.06.005. Epub 2014 Jul 5.
The risk of multiple pregnancy to maternal-fetal health can be minimized by reducing the number of embryos transferred. New tools for selecting embryos with the highest implantation potential should be developed. The aim of this study was to evaluate the ability of morphological and morphometric variables to predict implantation by analysing images of embryos. This was a retrospective study of 135 embryo photographs from 112 IVF-ICSI cycles carried out between January and March 2011. The embryos were photographed immediately before transfer using Cronus 3 software. Their images were analysed using the public program ImageJ. Significant effects (P < 0.05), and higher discriminant power to predict implantation were observed for the morphometric embryo variables compared with morphological ones. The features for successfully implanted embryos were as follows: four cells on day 2 of development; all blastomeres with circular shape (roundness factor greater than 0.9), an average zona pellucida thickness of 13 µm and an average of 17695.1 µm² for the embryo area. Embryo size, which is described by its area and the average roundness factor for each cell, provides two objective variables to consider when predicting implantation. This approach should be further investigated for its potential ability to improve embryo scoring.
通过减少移植胚胎的数量，可以将多胎妊娠对母婴健康的风险降至最低。应开发用于选择具有最高着床潜力胚胎的新工具。本研究的目的是通过分析胚胎图像来评估形态学和形态测量学变量预测着床的能力。这是一项对2011年1月至3月期间进行的112个体外受精-卵胞浆内单精子注射（IVF-ICSI）周期的135张胚胎照片进行的回顾性研究。在移植前立即使用Cronus 3软件对胚胎进行拍照。使用公共程序ImageJ对其图像进行分析。与形态学变量相比，形态测量学胚胎变量对预测着床具有显著影响（P < 0.05）和更高的判别能力。成功着床胚胎的特征如下：发育第2天有4个细胞；所有卵裂球呈圆形（圆度因子大于0.9），透明带平均厚度为13 µm，胚胎面积平均为17695.1 µm²。胚胎大小由其面积和每个细胞的平均圆度因子描述，在预测着床时提供了两个需要考虑的客观变量。这种方法因其改善胚胎评分的潜在能力应进一步研究。
