Garside W T, Loret de Mola J R, Bucci J A, Tureck R W, Heyner S
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104, USA.
Mol Reprod Dev. 1997 May;47(1):99-104. doi: 10.1002/(SICI)1098-2795(199705)47:1<99::AID-MRD13>3.0.CO;2-V.
Zona pellucida thickness was measured daily in zygotes and cleavage stage embryos. Measurements were performed on a Nikon inverted microscope equipped with Hoffman modulation optics, using an ocular micrometer. Zona thickness of each zygote/embryo was measured four times, the zygote/embryo was then "rolled over," and four more measurements were repeated for a total of eight. The zygotes/embryos were photographed daily and the measurements repeated on the prints. Subsequently, the mean zona thickness for each stage was calculated. A total of 81 patients (mean age 33.8 +/- 4.2) participated in the study. A total of 427 embryos were evaluated. Categorical data differences between groups were evaluated by ANOVA and multiple linear regression. For nominal data, the Kruskal-Wallis test was applied; when P < 0.05 the differences were considered to be significant. We found that the average zona thickness on day 1 of in vitro culture was 17.7 +/- 0.14 microns; 16.3 +/- 0.14 microns on day 2 and 14.9 +/- 0.14 microns on day 3 (P < .0001). When the zona thickness was analyzed in relation to the number of blastomeres on day 3 of culture, there was a highly significant correlation with blastomere number (P < .0001). Similarly, there was a highly significant correlation with embryo grade (P < .005) and fragmentation (P < .001). The data were also analyzed for embryos transferred that resulted in a successful pregnancy, revealing that embryos in a pregnancy cycle had significantly thinner zonae pellucidae (P < .0001), when compared to embryos that were not transferred or from nonconceptual cycles. The average zona thickness also decreased with age, and was most apparent after 35 years. Changes in zona thickness correlated with the number of blastomeres, grade, fragmentation, age and were more evident in embryos transferred from cycles resulting in successful pregnancies. Therefore, zona pellucida measurements should be included in the overall assessment of embryo quality, since this information may be useful in the selection of optimal embryos for transfer.
每天测量受精卵和卵裂期胚胎的透明带厚度。测量在配备霍夫曼调制光学系统的尼康倒置显微镜上进行，使用目镜测微计。每个受精卵/胚胎的透明带厚度测量四次，然后将受精卵/胚胎“翻转”，再重复测量四次，共测量八次。每天对受精卵/胚胎进行拍照，并在照片上重复测量。随后，计算每个阶段的平均透明带厚度。共有81名患者（平均年龄33.8±4.2岁）参与了该研究。共评估了427个胚胎。通过方差分析和多元线性回归评估组间分类数据差异。对于名义数据，应用Kruskal-Wallis检验；当P<0.05时，差异被认为具有统计学意义。我们发现，体外培养第1天的平均透明带厚度为17.7±0.14微米；第2天为16.3±0.14微米，第3天为14.9±0.14微米（P<.0001）。当分析培养第3天的透明带厚度与卵裂球数量的关系时，与卵裂球数量存在高度显著相关性（P<.0001）。同样，与胚胎等级（P<.005）和碎片率（P<.001）也存在高度显著相关性。还对导致成功妊娠的移植胚胎数据进行了分析，结果显示，与未移植胚胎或非妊娠周期的胚胎相比，妊娠周期的胚胎透明带明显更薄（P<.0001）。平均透明带厚度也随年龄增长而降低，35岁以后最为明显。透明带厚度的变化与卵裂球数量、等级、碎片率、年龄相关，并且在导致成功妊娠的周期中移植的胚胎中更为明显。因此，透明带测量应纳入胚胎质量的整体评估中，因为这些信息可能有助于选择最佳的移植胚胎。
