Fahrenkopf Max A, Mukherjee Tamal, Ydstie B Erik, Schneider James W
Department of Chemical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Electrophoresis. 2014 Dec;35(23):3408-14. doi: 10.1002/elps.201400266. Epub 2014 Oct 20.
We present a nonlinear optimization study of different implementations of the DNA electrophoretic method "End-labeled Free-solution Electrophoresis" in commercial capillary electrophoresis systems and microfluidics to improve the time required for readout. Here, the effect of electro-osmotic counterflows and snap-shot detection are considered to allow for detection of peaks soon after they are electorphoretically resolved. Using drag tags available in micelle form, we identify a design capable of sequencing 600 bases in 2.8 min.
我们对商业毛细管电泳系统和微流控技术中“末端标记自由溶液电泳”这种DNA电泳方法的不同实施方案进行了非线性优化研究，以缩短读出所需时间。在此，考虑了电渗逆流和快照检测的影响，以便在峰电泳分离后不久就能进行检测。使用胶束形式的拖尾标签，我们确定了一种能够在2.8分钟内对600个碱基进行测序的设计。
