Jones K R, Cottrell B J, Targett G A, Playfair J H
Department of Immunology, University College and Middlesex School of Medicine, London, UK.
Parasite Immunol. 1989 Nov;11(6):585-92. doi: 10.1111/j.1365-3024.1989.tb00922.x.
Freshly isolated human peripheral blood monocytes inhibited the growth of blood-stage asexual Plasmodium falciparum parasites in vitro. The monocytes contained intracellular parasite pigment and a few whole parasites, but the remaining parasites reinvaded fresh red cells successfully and were morphologically normal. Anti-parasitic activity of these macrophages was not significantly enhanced by treatment with recombinant tumour necrosis factor alpha, recombinant gamma-interferon or lymphoblastoid alpha-interferon. Catalase had no effect on this parasite inhibition, suggesting a hydrogen peroxide independent mechanism. Anti-parasitic activity was, however, enhanced by prior maturation of the monocytes. Monocytes matured for 6 days caused 100% killing of parasites. In contrast to identical concentrations of freshly isolated monocytes the parasites incubated with these matured macrophages showed intraerythrocytic death similar to the crisis forms seen in vivo. gamma-interferon present either during the assay or as a pretreatment had no significant enhancing effect on the killing, although cytotoxicity to tumour cell lines was enhanced. Conditioned medium from macrophages showed only moderate parasite inhibition.
新鲜分离的人外周血单核细胞在体外可抑制血液期恶性疟原虫无性体的生长。单核细胞内含有细胞内寄生虫色素和一些完整的寄生虫，但其余寄生虫成功重新侵入新鲜红细胞且形态正常。用重组肿瘤坏死因子α、重组γ干扰素或淋巴母细胞样α干扰素处理后，这些巨噬细胞的抗寄生虫活性并未显著增强。过氧化氢酶对这种寄生虫抑制作用没有影响，提示存在一种不依赖过氧化氢的机制。然而，单核细胞预先成熟可增强抗寄生虫活性。成熟6天的单核细胞可导致100%的寄生虫死亡。与相同浓度的新鲜分离单核细胞相比，与这些成熟巨噬细胞共同孵育的寄生虫显示出与体内所见危机形式相似的红细胞内死亡。检测期间存在的γ干扰素或作为预处理的γ干扰素对杀伤作用没有显著增强作用，尽管对肿瘤细胞系的细胞毒性有所增强。巨噬细胞的条件培养基仅显示出适度的寄生虫抑制作用。
