Gyan B, Troye-Blomberg M, Perlmann P, Björkman A
Department of Immunology, Stockholm University, Sweden.
Parasite Immunol. 1994 Jul;16(7):371-5. doi: 10.1111/j.1365-3024.1994.tb00362.x.
Adherent cells from human peripheral blood were studied for their interaction with asexual blood forms of Plasmodium falciparum in vitro. Freshly isolated monocytes only showed weak anti-parasitic effects. However, an enhancement of this anti-parasitic activity was apparent when monocytes were allowed to mature in vitro. Monocytes activated with IFN-gamma for two or three days had an enhanced anti-parasitic effect. In contrast, the inhibition mediated by cells incubated for five days was the same with or without IFN-gamma treatment. There was no evidence of toxicity when IFN-gamma at high concentrations was added directly to P. falciparum cultures. The anti-parasitic activity of the activated cells seemed to be due to nitric oxide since incubation of macrophages with L-NMMA reduced the level of inhibition. However, inhibition was only partial suggesting that other factors also were involved in inhibition of parasite growth.
对来自人外周血的贴壁细胞在体外与恶性疟原虫的无性血液形式的相互作用进行了研究。新鲜分离的单核细胞仅表现出较弱的抗寄生虫作用。然而，当单核细胞在体外成熟时，这种抗寄生虫活性会增强。用γ干扰素激活两天或三天的单核细胞具有增强的抗寄生虫作用。相比之下，培养五天的细胞介导的抑制作用在有无γ干扰素处理时是相同的。当将高浓度的γ干扰素直接添加到恶性疟原虫培养物中时，没有毒性证据。活化细胞的抗寄生虫活性似乎归因于一氧化氮，因为用L-NMMA孵育巨噬细胞会降低抑制水平。然而，抑制只是部分的，这表明其他因素也参与了寄生虫生长的抑制。
