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具有仿生微图案的表面可减少脓肿分枝杆菌的定植。

A surface with a biomimetic micropattern reduces colonization of Mycobacterium abscessus.

作者信息

Kim Eun, Kinney William H, Ovrutsky Alida R, Vo Danthy, Bai Xiyuan, Honda Jennifer R, Marx Grace, Peck Emily, Lindberg Leslie, Falkinham Joseph O, May Rhea M, Chan Edward D

机构信息

Department of Medicine and Academic Affairs, National Jewish Health, Denver, CO, USA.

出版信息

FEMS Microbiol Lett. 2014 Nov;360(1):17-22. doi: 10.1111/1574-6968.12587. Epub 2014 Sep 9.

Abstract

Nontuberculous mycobacteria (NTM) are ubiquitous organisms found in soil, water, and biofilms. Engineered surface topography has been proposed as a method to reduce microbial biofilm formation. The Sharklet(®) micropattern silicone surface has been shown to reduce biofilm formation of pyogenic bacteria. We hypothesized that this micropattern surface will also reduce colonization by Mycobacterium abscessus, a human pathogen. Smooth and micropattern silicone samples were incubated with 1 × 10(6) M. abscessus mL(-1) for 2 and 4 days. After processing to optimize recovery of adhered mycobacteria, there was a 75% and 50% reduction in the number of viable M. abscessus recovered from the micropattern surfaces compared to the smooth surfaces at 2 and 4 days after inoculation, respectively. Ziehl-Neelsen staining after measures to remove the adherent microorganisms revealed fewer residual M. abscessus on the micropattern samples as compared to smooth samples, validating the quantitative culture results. Microscopic observation of 2, 4, and 8 day M. abscessus cultures on micropattern samples showed that the organisms preferentially colonized within the channels between the rectangular features. In summary, a micropattern surface reduces the colonization of a pathogenic NTM. It remains to be seen whether this micropattern can reduce infections in humans.

摘要

非结核分枝杆菌(NTM)是存在于土壤、水和生物膜中的普遍微生物。工程化表面形貌已被提议作为一种减少微生物生物膜形成的方法。鲨纹(®)微图案硅胶表面已被证明可减少化脓性细菌的生物膜形成。我们假设这种微图案表面也会减少脓肿分枝杆菌(一种人类病原体)的定植。将光滑和微图案硅胶样品与1×10⁶CFU/mL的脓肿分枝杆菌一起孵育2天和4天。在经过处理以优化回收粘附的分枝杆菌后,与光滑表面相比,在接种后2天和4天从微图案表面回收的活脓肿分枝杆菌数量分别减少了75%和50%。在采取措施去除粘附微生物后进行的萋-尼染色显示,与光滑样品相比,微图案样品上残留的脓肿分枝杆菌更少,这验证了定量培养结果。对微图案样品上2天、4天和8天的脓肿分枝杆菌培养物进行显微镜观察表明,这些微生物优先在矩形结构之间的通道内定植。总之,微图案表面可减少致病性非结核分枝杆菌的定植。这种微图案是否能减少人类感染还有待观察。

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