禽分枝杆菌、胞内分枝杆菌和脓肿分枝杆菌对家用管道材料的黏附及生物膜形成。
Adherence and biofilm formation of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium abscessus to household plumbing materials.
机构信息
Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 USA.
出版信息
J Appl Microbiol. 2013 Sep;115(3):908-14. doi: 10.1111/jam.12272. Epub 2013 Jun 24.
AIMS
Measure adherence and biofilm formation by cells of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium abscessus on common household plumbing materials namely stainless steel, glass, zinc-galvanized steel, copper and polyvinyl chloride (PVC).
METHODS AND RESULTS
Coupons in a CDC biofilm reactor were exposed to cell suspensions containing 10(5) NTM colony forming units (CFU) per ml and adherence measured for 6 h. Biofilm formation (increased numbers of adherent CFU) was measured weekly to 21 days in the absence of substantial numbers of suspended mycobacterial cells. Adherence was rapid and substantial with 2000-15 000 CFU cm(-2) adhering within 1-6 h at room temperature. Biofilm numbers reached as high as 10(7) CFU cm(-2) . Biofilm-grown cells of Myco. avium were more adherent compared with suspension-grown cells.
CONCLUSIONS
Mycobacterium avium, Myco. intracellulare and Myco. abscessus readily adhered and formed biofilms on all types of plumbing materials. Factors influencing adherence and biofilm formation were species, plumbing material and prior growth.
目的
测量细胞分枝杆菌、细胞内分枝杆菌和脓肿分枝杆菌在常见家用管道材料（不锈钢、玻璃、镀锌钢、铜和聚氯乙烯（PVC））上的黏附和生物膜形成情况。
方法和结果
将 CDC 生物膜反应器中的小方片暴露于每毫升含有 10(5) 个非结核分枝杆菌集落形成单位（CFU）的细胞悬浮液中，并测量 6 小时的黏附情况。在没有大量悬浮分枝杆菌细胞的情况下，每周测量生物膜形成情况（附着 CFU 数量增加），直至 21 天。在室温下，1-6 小时内迅速且大量黏附，黏附的 CFU 数量达到 2000-15000 CFU cm(-2)。生物膜生长的细胞数量可高达 10(7) CFU cm(-2)。与悬浮生长的细胞相比，分枝杆菌属的生物膜生长的细胞更容易黏附。
结论
分枝杆菌、细胞内分枝杆菌和脓肿分枝杆菌容易在所有类型的管道材料上黏附和形成生物膜。影响黏附和生物膜形成的因素有物种、管道材料和先前的生长情况。
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