Sandle T, Vijayakumar R, Saleh Al Aboody M, Saravanakumar S
Bio Products Laboratory, Elstree, Hertfordshire, UK.
J Appl Microbiol. 2014 Nov;117(5):1267-73. doi: 10.1111/jam.12628. Epub 2014 Oct 3.
To determine the minimum inhibitory concentrations (MIC) of a range of cleanroom fungi against three disinfectants common to the pharmaceutical and healthcare sectors: biguanide (chlorhexidine) and two quaternary ammonium compounds (benzalkonium chloride and cetrimide).
The in vitro fungicidal activities of the three biocides were studied against 112 cleanroom fungal isolates using broth microdilution technique (CLSI M38-A2 standard).
Minimum inhibitory concentration (MIC) for all three biocides against hyaline fungi showed results of not more than 16 μg ml(-1). Alternaria showed <32 μg ml(-1) and other dematiaceous fungi reported that 8-16 μg ml(-1) for biguanides and QACs. This study clearly demonstrates that the most frequently isolated micro-organisms from an environmental monitoring programme may be periodically subjected to broth microdilution testing with cleanroom disinfectant agents used in the disinfection programme confirm their sensitivity profile.
No large collection of data exists on the activity of biocides on pharmaceutical cleanroom fungal isolates. This is the first study report with large collection of cleanroom fungal isolates tested against common biocides using the broth microdilution antifungal susceptibility testing to determine the MIC value. The data presented support a quality control procedure for cleanroom disinfection.
确定一系列洁净室真菌对制药和医疗保健行业常用的三种消毒剂的最低抑菌浓度（MIC）：双胍（洗必泰）和两种季铵化合物（苯扎氯铵和西曲溴铵）。
采用肉汤微量稀释技术（CLSI M38 - A2标准）研究了这三种杀菌剂对112株洁净室真菌分离株的体外杀菌活性。
所有三种杀菌剂对透明真菌的最低抑菌浓度（MIC）结果均不超过16μg/ml。链格孢菌显示<32μg/ml，其他暗色真菌报告双胍类和季铵化合物的MIC为8 - 16μg/ml。本研究清楚地表明，从环境监测计划中最常分离出的微生物可能需要定期用消毒程序中使用的洁净室消毒剂进行肉汤微量稀释测试，以确认其敏感性概况。
关于杀菌剂对制药洁净室真菌分离株活性的大量数据尚无报道。这是第一项使用肉汤微量稀释抗真菌药敏试验对大量洁净室真菌分离株进行测试以确定MIC值的研究报告。所提供的数据支持洁净室消毒的质量控制程序。
