评估霉菌分离株对杀菌剂的敏感性。

Assessment of susceptibility of mould isolates towards biocides.

作者信息

Kalkanci A, Elli M, Adil Fouad A, Yesilyurt E, Jabban Khalil I

机构信息

Department of Medical Microbiology, Gazi University School of Medicine, Besevler, Ankara, 06500, Turkey.

AAT-Advanced Analytical Technologies Srl, Piacenza, Italy.

出版信息

J Mycol Med. 2015 Dec;25(4):280-6. doi: 10.1016/j.mycmed.2015.08.001. Epub 2015 Sep 26.

DOI:10.1016/j.mycmed.2015.08.001

PMID:26420602

Abstract

OBJECTIVES

The aim of this study was to determine the susceptibility of 77 mould strains: Aspergillus fumigatus (20), Aspergillus flavus (8), Aspergillus niger (4), Aspergillus ochraceus (2), Penicillium citrinum (15), Penicillium crysogenum (14), Penicillium aurantiogriseum (1), Penicillium roquefortii (4), Penicillium paneum (2), Rhizopus spp. (3), Tricoderma spp. (1) and Mucor spp. (3) to biocides.

METHODS

MIC determination was determined based on CLSI methodology.

RESULTS

For hospital acquired strains, MIC50 was 0.5mg/L, MIC90 was 1mg/L for chlorhexidine (CHX); MIC50 was 0.5mg/L, and MIC90 was 1mg/L for benzalkonium chloride (BZC); MIC50 was 1mg/L, and MIC90 was 2mg/L for triclosan (TRC); MIC50 was 1024mg/L, and MIC90 was 2048mg/L for sodium hypochloride (SHC). For feed and food isolates MIC50 was 2mg/L, MIC90 was 8mg/L for CHX, MIC50 was 2mg/L, and MIC90 was 4mg/L for BZC, MIC50 was 2mg/L, and MIC90 was 4mg/L for TRC, MIC50 was 256mg/L, and MIC90 was 512mg/L for SHC.

CONCLUSION

We can conclude that food isolates presented slightly higher MIC50 and MIC90 values for CHX, BNZ and TRC, but not for SHC.

摘要

目的

本研究旨在测定77株霉菌菌株的药敏性，这些菌株包括烟曲霉（20株）、黄曲霉（8株）、黑曲霉（4株）、赭曲霉（2株）、桔青霉（15株）、产黄青霉（14株）、橙灰青霉（1株）、罗克福特青霉（4株）、潘氏青霉（2株）、根霉属（3株）、木霉属（1株）和毛霉属（3株）对杀菌剂的敏感性。

方法

根据临床和实验室标准协会（CLSI）方法测定最低抑菌浓度（MIC）。

结果

对于医院获得性菌株，洗必泰（CHX）的MIC50为0.5mg/L，MIC90为1mg/L；苯扎氯铵（BZC）的MIC50为0.5mg/L，MIC90为1mg/L；三氯生（TRC）的MIC50为1mg/L，MIC90为2mg/L；次氯酸钠（SHC）的MIC50为1024mg/L，MIC90为2048mg/L。对于饲料和食品分离株，CHX的MIC50为2mg/L，MIC90为8mg/L；BZC的MIC50为2mg/L，MIC90为4mg/L；TRC的MIC50为2mg/L，MIC90为4mg/L；SHC的MIC50为256mg/L，MIC90为512mg/L。