Sanda Minoru, Shiota Makoto, Fujii Masaki, Kon Kazuhiro, Fujimori Tatsuya, Kasugai Shohei
Department of Oral Implantology and Regenerative Dental Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Clin Oral Implants Res. 2015 Dec;26(12):1369-74. doi: 10.1111/clr.12473. Epub 2014 Aug 26.
The aim of this experimental study was to test a mixture of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) granules inserted in cranial defects in rabbits, by the evaluation of the hard tissues volume, new bone formation, and residual graft after 4 and 8 weeks.
Two defects of 8 mm diameter were created at the calvarial bone of 24 Japanese white rabbits for a total of 48 defects. Four groups were created: defects filled with a mixture of HA and beta-TCP granules (test A), defects filled with HA alone (test B), defects filled with beta-TCP (test C), and empty defects (control). Hard tissues volume (remaining graft + new bone) was evaluated by μ-CT and new bone (NB) and remaining graft (RG) percentages were evaluated by histomorphometry. The animals were sacrificed at 4 or 8 weeks postoperatively.
The test groups A, B, and C showed a significant higher total volume compared with controls at 4 and 8 weeks (P < 0.05). Regarding the percentages of NB and RG at 4 and 8 weeks, no significant differences were detected (P > 0.05). When comparing 4 and 8 weeks, test group A showed a significant increase in new bone formation. At both 4 and 8 weeks, no group showed significant differences in NB (P > 0.05). At 8 weeks, test group B had more RG than test group A.
The novel mixture could maintain the volume of the grafted area compared with that with intervention, and in a similar way compared with HA.
本实验研究旨在通过评估4周和8周后兔颅骨缺损处的硬组织体积、新骨形成情况及残余移植物，来测试植入兔颅骨缺损处的羟基磷灰石（HA）和β-磷酸三钙（β-TCP）颗粒混合物。
在24只日本白兔的颅骨上制造两个直径8mm的缺损，共48个缺损。分为四组：缺损处填充HA和β-TCP颗粒混合物（测试A组）、仅填充HA的缺损（测试B组）、填充β-TCP的缺损（测试C组）以及空白缺损（对照组）。通过μ-CT评估硬组织体积（残余移植物+新骨），通过组织形态计量学评估新骨（NB）和残余移植物（RG）的百分比。术后4周或8周处死动物。
测试A组、B组和C组在4周和8周时的总体积均显著高于对照组（P<0.05）。关于4周和8周时NB和RG的百分比，未检测到显著差异（P>0.05）。比较4周和8周时，测试A组新骨形成显著增加。在4周和8周时，各组NB均无显著差异（P>0.05）。在8周时，测试B组的RG比测试A组更多。
与干预组相比，这种新型混合物能够维持移植区域的体积，且与HA相比情况类似。
